Electronic Resource
Springer
Research in experimental medicine
189 (1989), S. 229-239
ISSN:
1433-8580
Keywords:
Scanning microfluorometry
;
Intravital microvascular research
;
Rats
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Our own development of fluorometric scanning techniques in intravital microscopy of the microcirculation is described. Very tiny amount of fluorometric substances are detected with a high temporal and locational resolution. The everted small intestinal mesentery of the rat serves as a model. We have given a detailed description of the microscopes used, the optical systems, the conditions of measurement of the microfluorometry, the scanning techniques and the evaluation of the measurement data. The present state of technical development detects 10−12 g of a fluorochromed plasma protein in 8 ms in a measurement field of 2 µm2. The four-digit measurement data of a scanning line of 200 µm length in 0.25 µm locational resolution are registered in about 2 s.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01852254
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