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  • Key words: Heterotopic osteoblast-like cells — Colony formation — Differentiation — Alkaline phosphatase — Osteocalcin.  (1)
  • Schlüsselwörter Fallpauschalen • Sonderentgelte • Bundespflegesatzverordnung • Krankenhausinformationssystem • Computergestützte Codierung  (1)
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  • 1
    ISSN: 1432-0827
    Keywords: Key words: Heterotopic osteoblast-like cells — Colony formation — Differentiation — Alkaline phosphatase — Osteocalcin.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract. In this study, a characterization of human bone-forming cells responsible for heterotopic ossification was carried out in vitro. The biological and biochemical cell characteristics of the heterotopic osteoblast-like (HOB) cells were compared with those of orthotopic osteoblast-like (OB) cells from normal bone and stromal bone marrow cells believed to contain a subpopulation of osteogenic precursor cells. We found that HOB's from the spongiosa of heterotopic ossification required less time until the beginning of migration and the achievement of confluence in vitro compared with OBs from femoral shaft spongiosa. The fraction of mitotically active cells assessed by a clonogenic assay was higher as well in HOB cells. The in vitro studies of mitogenesis and the efficiency of colony formation of osteogenic cells indicate that with increasing differentiation and relative age they become more dependent on growth factors in the medium, otherwise the morphology of osteoblast-like cells changes and they pass irreversibly into the postmitotic stage of the cell cycle. The activity of the alkaline phosphatase is distinctly higher in the HOB than in the OB cells, HOB cells exhibit a lower level of osteocalcin expression compared with OB cells. No significant difference was found between OB and HOB cells in the amount of procollagen of type I sequestered by the cells. After 30 days, HOB and OB cells formed a mineralized matrix on exposure to 2 mM β-glycerophosphate. Since HOBs were isolated from heterotopic bone that had developed within 3–6 months after hip surgery, the differences in cellular behavior compared with OBs may be attributed to the relatively young age of HOB cells.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1433-044X
    Keywords: Key words Diagnosis-related billing • Payment for special procedures • Health maintenance law • Computer-assisted documentation ; Schlüsselwörter Fallpauschalen • Sonderentgelte • Bundespflegesatzverordnung • Krankenhausinformationssystem • Computergestützte Codierung
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Nach dem Gesundheitsstrukturgesetz besteht für alle deutschen Kliniken seit dem 01. 01. 1995 die Verpflichtung zur Dokumentation und Kodierung von Einweisungs-, Aufnahme- und Entlaßdiagnose in die ICD-9 und die Dokumentation und Verschlüsselung zumeist operativ erbrachter Leistungen in die ICPM. Seit dem 01. 01. 1996 besteht zudem die Verpflichtung zur Abrechnung nach leistungsabhängigen vollpauschalierten Entgeltformen (Fallpauschalen und Sonderentgelte), wobei sich der Abrechnungsentscheid auf die erhobenen Daten stützt. In der Berufsgenossenschaftlichen Unfallklinik Tübingen wurden während des gesamten Jahres 1996 alle Diagnosen und Therapien handschriftlich dokumentiert und mit Hilfe zweier Handbücher kodiert. Seit 01. 01. 1997 wird ausschließlich rechnergestützt dokumentiert und kodiert. Die Entscheidung über die Abrechnungsform wird anhand der eingegebenen Daten vom Klinikinformationssystem vorgeschlagen. Retrospektiv wurden anhand von Krankenakten und Abrechnungsbelegen jeweils die Monate Januar bis April der Jahre 1996 und 1997 auf die Anzahl der abgerechneten Fallpauschalen und Sonderentgelte hin verglichen. Hierbei zeigt sich, daß durch eine rechnergestützte Kodierung und Dokumentation die Anzahl der erkannten und tatsächlich abgerechneten Fallpauschalen und Sonderentgelte im Vergleich zum Vorjahr deutlich zugenommen hat.
    Notes: Summary Since 1995 German health maintenance laws require hospitals to document and code all referals, admissions and discharges using the 4-digit ICD. Operative procedures are documented and coded using the ICPM. Beginning in January 1996, reimbursement for health services requires a diagnosis-related billing and payment for special procedures. The decision for billing is based on documented diagnosis and therapy. This extended request for documentation makes an online access to diagnosis and therapy with a computer-assisted coding system advisable. In 1996 in our hospital each diagnosis and operation was manually documented and coded on a form. Since the beginning of 1997, documentation and coding has been exclusively computer-assisted. On the basis of documented diagnosis and therapy the computer provides the route of reimbursement. Retrospectively we evaluated the number of charged diagnosis-related billings and payments for special procedures from January to April of 1996 and 1997. It became evident that with computer-assisted documentation and coding the number of detected and charged diagnosis-related billings and payments for special procedures was significantly increased in comparison with the previous year.
    Type of Medium: Electronic Resource
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