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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Genetica 97 (1996), S. 65-72 
    ISSN: 1573-6857
    Keywords: Secale cereale ; rye ; B chromosomes ; inbred lines ; pairing ; pollen mitosis ; transmission genotypes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract B chromosomes from an experimental population of the Japanese JNK strain of rye, isogenic for its Bs, have been backcrossed into twelve different inbred lines. The experiment is a way of studying the effects of the Bs against a range of different homozygous A chromosome backgrounds. This publication deals with pairing effects of both the As and the Bs, and their interactions, and with pollen mitosis. At meiosis there is a genotypic component to B effects, and they do not appear to act solely through a physical disturbance within the nucleus. In pollen the Bs are always present in more than 50% of the grains regardless of their pairing behaviour during meiosis; this result fits with a ‘parasitic’ model of the activity of rye Bs.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-6849
    Keywords: B chromosome ; DNA sequence composition ; fluorescencein situ hybridization ; repetitive sequence ; Secale cereale ; translocations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fluorescencein situ hybridization (FISH) has been used to analyse the structure of the rye B chromosome. Genomicin situ hybridization (GISH) demonstrates the high level of overall similarity between A and B chromosomes of rye, as well as the presence of a number of specific sequences. The B-specific repeat families D1100 and E3900 have been analysed in terms of their physical location and possible contiguity. Rye Bs contain members of the rye-specific dispersed repetitive family R173, as well as centromeric regions similar to those of the As. The B chromosomes analysed in our study lack detectable rDNA sequences. Anomalous results have been obtained with a number of subtelomeric repetitive probes from rye. Bs usually lack these sequences, but evidence is presented that in some cases A–B translocation events may relocate such sequences from the As to the Bs. These data are discussed in the context of current models for the origin of the B chromosome.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-6849
    Keywords: B chromosomes ; genomicin situ hybridization ; nuclear architecture ; Secale cereale ; Triticum aestivum cv ; Lindström
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The B chromosomes (Bs) of rye (Secale cereale) have been studied at interphase in terms of their chromatin organization, patterns of nuclear disposition and physical association in plants with two, three, and four Bs. The study was made in the Lindström strain of hexaploid wheat, which carries the rye Bs as an addition line, byin situ hybridization with a B-specific probe and by genomicin situ hybridization (GISH) with rye genomic DNA, enabling whole chromosome painting. Repetive sequences common to the As and Bs of rye allow for visualization of the rye B at interphase in the wheat background. A B-specific probe enables the orientation of two or more Bs to be determined, and the combination of both probes used together gives information on the disposition of the Bs and on their patterns of physical association within the nucleus. The Bs form linear ‘strings’, and the ends of their long arms, which can be detected by the B-specific probe, are usually located within the hemisphere of the nucleus that has the least condensed chromatin. There is dose-dependent association, and even numbers (2B, 4B) have a greater preference for association than odd ones (3B).
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Chromosome research 5 (1997), S. 177-181 
    ISSN: 1573-6849
    Keywords: B chromosomes ; interphase chromosome arrangement ; meiosis ; Secale cereale ; Triticum aestivum cv. Lindstro¨m
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Probes for B chromosome-specific sequences in the distal region of the long arm of the rye B have been used to investigate the interphase arrangement of the Bs in rye and in hexaploid wheat. The Lindstro¨m strain of wheat carries the rye Bs as additions. The number of in situ signals in nuclei with two, three and four Bs is often less than the maximum B number, and it seems that the Bs may be grouped together in various ways rather than being randomly dispersed throughout the nucleus. The degree of physical association is greater in rye than in the alien wheat background. The results are discussed in relation to the pairing and recombination preferences of the Bs in rye and in Lindstro¨m wheat.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Genetica 92 (1994), S. 149-154 
    ISSN: 1573-6857
    Keywords: Secale cereale ; rye ; B-chromosomes ; fitness ; inbred lines ; parasitic Bs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract B-chromosomes from an experimental population of the Japanese JNK strain of rye, isogenic for its Bs, have been backcrossed into twelve different inbred lines. The experiment provides a way to study the effects of the Bs against a range of homozygous A-chromosome backgrounds. This publication deals with vigour and fertility: it shows that the rye Bs fit a parasitic model, and that they interact in their effects with the A-chromosome background genotype.
    Type of Medium: Electronic Resource
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