ISSN:
0021-9541
Schlagwort(e):
Life and Medical Sciences
;
Cell & Developmental Biology
Quelle:
Wiley InterScience Backfile Collection 1832-2000
Thema:
Biologie
,
Medizin
Notizen:
Sugar deprivation of hamster fibroblasts (NILAbbreviations: ATP, ADP and AMP, adenosine 5′-tri-, di- and monophosphate respectively; CTP, CDP and CMP, cytosine 5′-tri-, di and monophosphate respectively; GTP, GDP and GMP, guanosine 5′-tri-, di- and monophosphate respectively; IMP, inosine 5′-monophosphate; Gal-1-P, α-D-galactose-1-phosphate; UDP-Gal, uridine 5′-diphosphogalactose; D-MEM, Dulbecco's modified Eagle's minimal essential medium; D-PBS, Dulbecco's phosphate-buffered saline (pH 7.2); NIL, a line of Syrian hamster fibroblasts; PyNIL, a polyoma virus-transformed line of NIL cells.) affected the steady state levels (pool sizes) of cellular acid soluble nucleotides in the following fashion; the pools of UTP, GTP and CTP decreased to a much greater extent than the cellular ATP pools, with the UTP pools undergoing the most dramatic reduction. Sugar deprivation of polyoma-transformed NIL cells (PyNIL) yielded even sharper decreases in the nucleoside triphosphate pools with relative changes similar to those of the untransformed cells. Inhibition of protein synthesis by cycloheximide, initiated at the onset of (and continued during) sugar deprivation, prevented the reduction in pool sizes and yielded values slightly higher than those observed for pool sizes in cells cultured in sugar-supplemented medium. Refeeding glucose to sugar-depleted hamster fibroblasts led to rapid increases (within 1 hour) in the UTP and CTP pools to levels well above the pool sizes observed in cells which were continuously cultured (16 hours) in sugar supplemented medium. Feeding NIL or PyNIL cells with fructose instead of glucose as the only hexose source did not appreciably affect any of the ribonucleoside triphosphate pool sizes. Measurements of hexose uptake by NIL and PyNIL cells under a variety of conditions suggest that hexose transport is not regulated by the total cellular pools of ATP or any of the other ribonucleoside triphosphates.
Zusätzliches Material:
3 Tab.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1002/jcp.1041010205
Permalink
