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  • 1985-1989  (2)
  • 1960-1964
  • Rhizobium phaseoli  (1)
  • Small bones  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    International orthopaedics 11 (1987), S. 277-282 
    ISSN: 1432-5195
    Keywords: Methyl methocrylate Heat of polymerisation ; Small bones
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Le but de ce travail est d'étudier les effets thermiques de la polymérisation sur les petits os longs. Les résultats montrent seulement, durant la prise du ciment, une élevation modérée de la température dans la cavité médullaire de ces os. L'élévation maximale (11° 7) est dans les limites de tolérance des cellules osseuses vivantes. Il a été démontré que les petites quantités de méthyl-métacrylate refroidissent plus rapidement, donc que diminuent davantage les effets nuisibles de la chaleur. Les examens histologiques ne montrent aucune altération due aux effets thermiques de la polymérisation du méthyl-métacrylate sur les petits os longs ni sur les tissus de recouvrement.
    Notes: Summary This study was designed to investigate the thermal effects of polymerization on small tubular bones. The results demonstrated only a slight increase in temperature during cement-curing in the medullary canals of these bones. The maximum rise (11.7° C) was within the levels of tolerance for living bone cells. It was demonstrated that the small volumes of methyl-methacrylate cool more rapidly, hence further decreasing the deleterious effects of heat. The histological studies showed no recognisable adverse reaction to the thermal effects of methyl-methacrylate polymerization on small tubular bones or on the overlying tissue.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1617-4623
    Keywords: Gene regulation ; Melanin synthesis ; Nitrogen fixation ; Phaseolus beans ; Rhizobium phaseoli
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The symbiotic plasmid pRP2JI of Rhizobium phaseoli strain 8002 was shown to contain two separate regions of DNA which are required and sufficient for the synthesis of the pigment melanin. One of these regions containing the class II mel gene(s) was located to other genes involved in nodulation and in nitrogen fixation. Mutations in this region abolished both the ability to synthesize melanin and to fix nitrogen in Phaseolus bean root nodules. Mutations in the other, unlinked region, containing class I mel gene(s), also abolished melanin synthesis but did not affect symbiotic nitrogen fixation. Transcriptional fusions between the class I mel gene and the Escherichia coli lacZ gene were constructed and it was demonstrated that the class II mel gene(s) activated their transcription in free-living culture. Further, strains containing the cloned regulatory class II gene(s) synthesized melanin when growing in minimal medium, in contrast to wild-type strains which became pigmented only in complete medium containing yeast extract and tryptone. It was shown by hybridization experiments that the regulatory mel gene was closely linked to or may correspond to the regulatory nifA gene; a fragment of R. phaseoli DNA which included the class II gene(s) of R. phaseoli hybridized to a previously identified nifA-like gene of R. leguminosarum, the species that nodulates peas.
    Type of Medium: Electronic Resource
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