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  • Articles: DFG German National Licenses  (19)
  • Chemistry  (16)
  • Somatic hybrid  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Somatic hybrids between Brassica juncea (L). Czern. and Diplotaxis harra (Forsk.) Boiss and the generation of backcross progenies (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 1167-1172 
    Details
    ISSN: 1432-2242
    Keywords: Protoplast fusion ; Somatic hybrid ; Brassica juncea ; Diplotaxis harra ; Wild gene transfer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An attempt to transfer genes from droughttolerant Diplotaxis harra, a wild relative of Brassica species, to an elite oil-yielding cultivar, B-85, of mustard (Brassica juncea) was made through protoplast fusion, as the two plant systems are sexually incompatible. By following the standard protocol for PEG-mediated protoplast fusion followed by high pH, high Ca++, DMSO treatment and appropriate cell-culture technique, 16 presumptive somatic hybrid plants could be regenerated. Chromosomal analysis of four such somatic hybrids revealed that three of them were asymmetric. Analysis of morphological characters, meiotic chromosomes, and esterase isoenzyme pattern revealed that all the somatic hybrids were different from each other. Furthermore four chromosomes of each genome could undergo homoeologous pairing at meiosis indicating the possibilities for genetic recombination and chromosomal rearrangements. Irregular distribution of chromosomes at anaphase-II at meiosis has been a consistent feature of these plants. Eventually, pollen of all the somatic hybrids showed complete infertility preventing the recovery of any selfed seed. Nevertheless, ovule fertility of one somatic hybrid was not totally impaired as it had set some seeds upon backcrossing with the B. juncea parent. The esterase isoenzyme banding pattern of 24 individual progeny plants of this backcross provided evidence for their recombinant nature. It was thus confirmed that a transfer of genetic traits from Diplotaxis harra to B. juncea had indeed taken place. Furthermore, it was conceptualised that a transfer of alien genes through the protoplast-fusion technique is primarily possible in situations where meiotic pairing of the chromosomes of the two participating genomes generates recombinant gametocytes which can pass through subsequent filial generations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223936
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  • 2
    Electronic Resource
    Electronic Resource
    “Erussica”, the intergeneric fertile somatic hybrid developed through protoplast fusion between Eruca sativa Lam. and Brassica juncea (L.) Czern. (1990)
    Springer
    Theoretical and applied genetics 79 (1990), S. 561-567 
    Details
    ISSN: 1432-2242
    Keywords: Intergeneric somatic hybridization ; Brassica + Eruca ; Somatic hybrid ; Partial somatic hybrid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Hypocotyl calli-derived protoplasts of two cultivars of Brassica juncea (2n=36), a major oil-seed crop, were fused with normal as well as γ-irradiated mesophyll protoplasts of Eruca sativa (2n=22). The irradiation of the Eruca fusion partner increased the plating efficiency as well as the morphogenic potentiality of the fusion products over the normal fusion. Fertile plants could be regenerated from such fusion products. Analysis of 63 out of 181 plants regenerated showed that, indeed, 11 somatic hybrids (2n=58) and 10 partial somatic hybrids (chromosome number ranged between 50 and 56) had been obtained. Pollen viability (0%–82.9%) and seed set (0%–50%) of the hybrids indicated them to be useful for future studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226168
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  • 3
    Electronic Resource
    Electronic Resource
    Intergeneric somatic hybrid production through protoplast fusion between Brassica juncea and Diplotaxis muralis (1988)
    Springer
    Theoretical and applied genetics 76 (1988), S. 915-922 
    Details
    ISSN: 1432-2242
    Keywords: Intergeneric somatic hybridisation ; Brassica ; Diplotaxis ; Somatic hybrid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The need to transfer genetic traits from Diplotaxis muralis (2n=42) to Brassica juncea (2n=36), a major oil seed crop of the tropical world, was realised. Since the two plant types are sexually incompatible, attempts were made to evolve parasexual hybrids as the result of protoplast fusion. Protoplasts of hypocotyl-derived calli of two cultivars of B. juncea were fused with normal and γ-irradiated mesophyll protoplasts of Diplotaxis muralis. Regeneration of 110 plants from the fused products was successfully achieved. Upon analysis of some of them, we realised that true somatic hybrids and partial somatic hybrids had been generated. Thus the primary goal of evolving intergeneric hybridisation products between these two plant types was fulfilled.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00273681
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  • 4
    Electronic Resource
    Electronic Resource
    Electroinitiated polymerization of halophenols: Electroinitiation potential-lumo relations (1988)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part B: Polymer Physics 26 (1988), S. 2485-2498 
    Details
    ISSN: 0887-6266
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Electroinitiated polymerizations can be carried out by direct electron transfer via constant potential electrolysis.1-3 In doing so, the anodic (Ep, a) and the cathodic (Ep, c) potentials of the monomers must be measured by cyclic voltammetry. The cathodic peak potentials of halophenols were measured on Pt0 electrode versus Ag0/Ag+ in acetonitrile.Electroinitiated polymerization of bis(pyridine), bis(4-chloro-2,6-dibromophenoxide) copper (II) complex was studied in dimethylformamide-tetrabutylammonium fluoroborate by direct electron transfer from the cathode. Poly(phenylene oxides) obtained from the anolyte revealed that the monomer undergoes 1,2 as well as 1,4 addition reactions.Lowest unoccupied molecular orbital energies of the monomers were calculated by Hückel molecular orbital theory. The experimental Ep, c values were correlated to theoretical LUMO energies and electron densities using a multivariable linear regression model.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/polb.1988.090261207
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  • 5
    Electronic Resource
    Electronic Resource
    Infrared spectra of acrylonitrile grafted jute fibers (1989)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 38 (1989), S. 1951-1955 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1989.070381016
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  • 6
    Electronic Resource
    Electronic Resource
    Infrared spectra of jute stick and alkali-treated jute stick (1991)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 42 (1991), S. 2943-2950 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Defatted jute stick was treated with sodium hydroxide solution (2% w/v) at ambient temperature (∼ 32∼C) and boiling water bath temperature (95-97°C). Infrared (IR) spectra of defatted jute stick and alkali-treated jute stick were studied. The IR spectra of the alkalitreated jute stick were conspicuous by the absence of the 1730 cm-1 band, as compared to that of jute stick. Another characteristic feature of the alkali treated jute sticks is the absence of the band at 1240 cm-1, which is replaced by the 1265 and 1225 cm-1 bands; other bands in the above samples also recorded significant changes.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1991.070421111
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  • 7
    Electronic Resource
    Electronic Resource
    Infrared spectra of jute stick treated with white-rot fungus (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 44 (1992), S. 1607-1613 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Jute stick was treated with white-rot fungus for incubation periods of 6 days (MBA) and 12 days (MBB). The infrared spectra of fungus treated samples (MBA and MBB) and control jute stick (MBC) were analyzed and compared. The bands attributed mainly to hemicellulose show an increase in absorbance intensity ratios (Aν/A2900) with increase of incubation time. Similarly the bands attributed to lignin show an increase in the absorbance intensity ratios with increase of incubation time. Increase in the intensity of 1635 cm-1 band with increase of incubation time was also observed.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1992.070440914
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  • 8
    Electronic Resource
    Electronic Resource
    Infrared spectra of jute stick bleached with sodium chlorite and hydrogen peroxide (1991)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 43 (1991), S. 2187-2192 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Jute stick was bleached with sodium chlorite and alkaline hydrogen peroxide solutions. The infrared (IR) spectra of bleached samples and jute stick were analyzed and compared. The bleached samples were characterized by higher absorbance intensity ratios (Av/A2900) of the bands attributed to hemicellulose. Among the bleached samples the chlorite-bleached sample showed higher intensity for the bands mentioned. The bands attributed to lignin are either absent or very weak in the chlorite-bleached jute stick as compared to that of peroxide-bleached jute stick, although some residual lignin was present in the substrate. The 1635 cm-1 band attribute to the vibration of adsorbed water molecules in the noncrystalline regions in cellulose appears as a sharp peak in chlorite-bleached jute stick and as a shoulder in peroxide-bleached jute stick. This can be attributed to the difference in the nature of the two bleaching processes.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1991.070431205
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  • 9
    Electronic Resource
    Electronic Resource
    Infrared spectra of alkali treated jute stick (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 41 (1990), S. 329-336 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The infrared spectra of alkaline jute stick have been discussed and compared to that of untreated and control sample. A higher shift in the O—H and lower shift in the C—H stretching frequencies were observed in alkaline sample. The presence of a strong band at 1440-1430 cm-1 may be due to the coupling of COO- frequency with CH2 deformation frequency. The C—O stretching mode at 1240 cm-1 disappears on alkaline treatment with the formation of new peaks at 1265 and 1225 cm-1. The β-glucosidic band at 895 cm-1 is shifted to 880 cm-1 with doubled intensity in alkaline jute stick.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1990.070410125
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  • 10
    Electronic Resource
    Electronic Resource
    Characterization of grafted jute fiber by optical and scanning electron microscopy (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 49 (1993), S. 25-30 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Raw and partially delignified jute fibers were grafted with acrylonitrile and methyl meth-acrylate monomers, using the redox system of initiation with ceric ammonium sulfate. Grafting was also carried out in a mixed monomer system with acrylonitrile and methyl methacrylate monomers in different proportions. The optimum grafting condition depends on the duration of grafting treatment and proportion of grafting monomers. The monomers entered the fiber matrix with high add-on and, at still higher graft add-ons, the ultimate fibers suffered disintegration. The surfaces were increasingly covered with grafted materials and longitudinal cracks developed on the fiber surface with higher add-on. © 1993 John Wiley & Sons, Inc.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1993.070490104
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