ISSN:
0749-1581
Schlagwort(e):
Nuclear Overhauser effect
;
Potein-ligand interaction
;
Spin diffusion Prostaglandin
;
2α
;
Bound state conformation
;
Time saving strategy
;
t1 streak subtraction
;
NOESY NAD+
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Quelle:
Wiley InterScience Backfile Collection 1832-2000
Thema:
Chemie und Pharmazie
Notizen:
Pure absorption phase two-dimensional nuclear Overhauser spectrocopy (NOESY), using a short cycle time protocol, has been used to study cross-relaxation between protons of ligands bound to proteins. The 2D experiment is an accurate and efficient method for determining exchange transferred NOEs. Truncated NOESY data sets (obtained in less than 3 h) for the test systems - prostaglandin F2α bound to albumin and NAD+ bound to three different dehydrogenase enzymes - provide NOE estimates within experimental error of those obtained by the more time-consuming one-dimensional experiments. The 2D data matrix has within it spin-diffusion controls and cross-relaxation spectra useful for the subtraction of unwanted signals including spin transfer from coincidentally perturbed protein resonances and t1 streaks and ridges.
Zusätzliches Material:
10 Ill.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1002/mrc.1260251202
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