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  • 1
    Electronic Resource
    Electronic Resource
    Culturability and survival of an extreme thermophile isolated from deep-sea hydrothermal vents (1996)
    Springer
    Archives of microbiology 166 (1996), S. 64-67 
    Details
    ISSN: 1432-072X
    Keywords: Key wordsThermococcus peptonophilus ; Deep-sea ; Hydrothermal vents ; Survival ; Extreme thermophiles ; Culturability ; Active cells ; Mortality ; Dead cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The culturability of a strictly anaerobic, extremely thermophilic archaeon, Thermococcus peptonophilus (optimal growth temperature: 85° C), was studied during survival stages at various temperatures (98, 85, 70, and 4° C). Total cell number (determined by DAPI staining), active cells (rhodamine-stained cells), and culturable cells (using most-probable-number) were counted over time. The number of culturable cells decreased under each condition tested. The total number of cells significantly decreased only at temperatures close to the maximum for growth (98° C); at this temperature, the cells spontaneously lysed. Our results suggested that survival at 4° C in oxygenated waters might be a mechanism for the dispersion of extreme thermophiles in the ocean. In addition, we proved the existence of T. peptonophilus cells in several physiological states: culturable cells, active non-culturable cells, inactive non-culturable cells, and dead cells. Cell death was caused by cellular lysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050356
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  • 2
    Electronic Resource
    Electronic Resource
    Pressure and temperature effects on growth and viability of the hyperthermophilic archaeon Thermococcus peptonophilus (1997)
    Springer
    Archives of microbiology 168 (1997), S. 1-7 
    Details
    ISSN: 1432-072X
    Keywords: Key wordsThermococcus peptonophilus ; Extreme thermophiles ; Large-scale cultivation ; Hydrostatic ; pressure ; Barophiles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We studied the effects of high temperatures and elevated hydrostatic pressures on the physiological behavior and viability of the extremely thermophilic deep-sea archaeon Thermococcus peptonophilus. Maximal growth rates were observed at 30 and 45 MPa although no significant increases in cell yields were detected. Growth at 60 MPa was slower. The optimal growth temperature shifted from 85° C at 30 MPa to 90–95° C at 45 MPa. Cell viability during the stationary phase was also enhanced under high pressure. A trend towards barophily at pressures greater than those encountered in situ at the sea floor was demonstrated at increasing growth temperatures. The viability of cells during starvation, at high temperature (90, 95° C), and at low temperature (10° C) was enhanced at 30 and 45 MPa as compared to atmospheric pressure. These results show that the extremely thermophilic archaeon T. peptonophilus is a barophile.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050462
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Immediate full-thickness facial defects reconstruction using the radial forearm free flap (1993)
    Springer
    European journal of plastic surgery 16 (1993), S. 287-291 
    Details
    ISSN: 1435-0130
    Keywords: Squamous cell carcinoma ; Full-thickness defects ; Reconstruction ; Free radial forearm flaps
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The restoration of composite tissue defects of the oral cavity involving skin and mucosa remains a difficult problem for reconstructive surgeons. A method for immediate one-stage reconstruction of combined intraoral and facial defects with the radial forearm free flap is described. Four patients operated on between July and December of 1991 for advanced squamous cell carcinoma of oral cavity involving oral mucosa, skin and in two cases the mandible were reconstructed with a radial forearm flap to obtain reconstruction of facial and intraoral mucosa. The area of the fold is deepithelialized to be sutured to adjacent tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00210569
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Reconstruction of maxillary and palatal defects with the free radial forearm flap (1995)
    Springer
    European journal of plastic surgery 18 (1995), S. 220-225 
    Details
    ISSN: 1435-0130
    Keywords: Squamous cell carcinoma ; Palate-Reconstruction-Forearm flap
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We present six patients with maxillary and palate defects that were reconstructed with the radial forearm flap. Four patients had malignant neoplasms involving the maxilla, three with squamous cell carcinoma and the fourth with recurrent basal cell carcinoma. They were treated with excision and immediate reconstruction using a radial forearm free flap. The other two patients presented with large fistulae between the maxilla and nasal sinuses, these being sequelae of previous surgical treatment for malignancies. The fistulae were closed with radial forearm free flaps. This method provides primary wound healing, restoration of palatal function, preservation of facial contour, and a minimal morbidity while obviating the need for palatal prosthesis. In the six cases, the oral cavity has been completely separated from the paranasal sinus and nasal cavity, and all patients demonstrated satisfactory deglutition and intelligible speech.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00178737
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    Paper (German National Licenses)
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