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  • 1
    Electronic Resource
    Electronic Resource
    A region of heterogeneity adjacent to the 5s ribosomal RNA gene of cereal rusts (1992)
    Springer
    Current genetics 22 (1992), S. 101-105 
    Details
    ISSN: 1432-0983
    Keywords: Intergenic region ; Stem rusts ; Puccinia graminis ; Leaf rusts ; Cereals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Total genomic DNA was isolated from three cereal stem rusts, Puccinia graminis f. sp. tritici, f. sp. secalis, f. sp. avenae, and two cereal leaf rusts, P. recondita f. sp. tritici and P. coronata f. sp. avenae, and analyzed for the presence of heterogeneity in the intergenic region of the ribosomal DNA repeat unit. A 1 kb region of the repeat unit between the 26s and the 5s rRNA genes (IGR-1) was amplified by PCR and was found to be heterogeneous within each isolate and variable in size between races and species. The PCR results were confirmed by Southern blot analysis of native DNA. In an isolate of race C36(48), heterogeneity appeared to be due to variable numbers of 0.1 kb subrepeats in IGR-1. Nine wheat stem rust strains representing nine different races produced a unique pattern of heterogeneity while two different isolates of one race were identical, as were five of another. This may provide a rapid method for race identification in wheat stem rust. Heterogeneity and polymorphism in rye stem rust, oat stem rust, wheat leaf rust, and oat crown rust, was less pronounced than in wheat stem rust. In the course of this work, the 5s rRNA gene was located and its position and orientation within the ribosomal repeat unit was established.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00351468
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Double-stranded RNAs in mitochondrial extracts of stem rusts and leaf rusts of cereals (1989)
    Springer
    Current genetics 15 (1989), S. 161-166 
    Details
    ISSN: 1432-0983
    Keywords: Double-stranded RNA ; dsRNA ; Mitochondria ; Stem rusts ; Leaf rusts ; Cereals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Double-stranded ribonucleic acids (dsRNA) were isolated from mitochondria in urediosporelings of three cereal stem rusts, Puccinia graminis f. sp. tritici, f. sp. secalis and f. sp. avenae, and two cereal leaf rusts, P. recondita f. sp. tritici and P. coronata f. sp. avenae, and analyzed by agarose gel electrophoresis. The double strandedness of the RNA molecules was characterized by nuclease treatments (RNase A, DNase 1 and S1 nuclease) and CF-11 cellulose column chromatography. No interspecific variation in multisegments of dsRNA was observed among races of each forma specialis. As to the interspecific variation, although each of three forma specialis of Puccinia graminis had similar dsRNA segments, 4.8, 5.0 and 5.2 kb, wheat leaf rust and oat crown rust had additional dsRNA segments of 2.7, 2.8, 5.8 and 6.0 kb. The presence of a dsRNA segment of 5 kb size in all isolates and species examined indicates that this unique segment can be a molecular marker for the rust family, Uredinales. Dot-blot hybridization indicated that there is no sequence homology between dsRNA segments and mitochondrial DNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00435464
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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