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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 41 (1985), S. 623-625 
    ISSN: 1420-9071
    Keywords: Stable fly ; Stomoxys calcitrans ; diflubenzuron ; tunicamycin ; N-acetylglucosaminyl transferase ; prepupae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Tunicamycin, an antibiotic, and diflubenzuron, an insect growth regulator, were tested to determine their effects on N-acetylglucosaminyl transferase fromS. calcitrans prepupae. Diflubenzuron had no effect, but tunicamycin inhibited the transfer of GlcNAc-1-P from UDP-GlcNAc to dolicholmonophosphate with an I50 of 1.5–4 ng/ml.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 21 (1995), S. 571-590 
    ISSN: 0271-2091
    Keywords: CDF ; predictions ; laser Doppler anemometry ; inlet port ; cylinder ; turbulence ; steady flow ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: This paper presents a combined experimental and computational study of the steady flow through an internal combustion engine inlet port. The port was of generic design with a straight centreline. The three-dimensional velocity and turbulence fields in the port and cylinder were simulated using a computational fluid dynamics programme. Laser sheet flow visualization and laser Doppler anemometry were also employed to investigate the flows and assess the predictions. The results show that a large-scale flow structure is created in the cylinder by the inlet jet and its interaction with the valve and cylinder walls. Both predictions and measurements show that the flow is strongly dependent on the valve lift but is not affected by the flow rate. Comparisons of the numerical predictions with the experimental data indicated that the mean flow features are accurately predicted in many parts of the flow field; some discrepancies are evident and stem primarily from the failure of the simulation to predict a small recirculation region in the port which affects the trajectory of the annular jet entering the cylinder. Calculations were also made without modelling the port shape by using simplified inlet conditions upstream of the valve seat. It was found that this approximation can provide a reasonable, albeit less accurate, description of the flow, but modelling of the port shape is necessary for accurate flow predictions.
    Additional Material: 18 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 23 (1996), S. 1085-1109 
    ISSN: 0271-2091
    Keywords: intake port ; dual intake ; CFD ; laser Doppler anemometry ; internal combustion engine ; cylinder ; turbulence ; steady flow ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The influence of intake port design on the flow field in a dual-intake valve engine was investigated using computational fluid dynamics, in order to study the effect of inlet port design on the in-cylinder flow. A detailed 3D computational grid incorporating all the features of the Ford Zetec production engine inlet ports, valves and cylinder head was initially created and the flow structure modelled at 5 and 10 mm valve lifts under steady flow conditions. Comparisons of computational results with experimental data obtained by laser Doppler anemometry indicate that the flow characteristics have been predicted well in most regions. Flow generated by different intake port designs was also simulated by introducing air into the cylinder at different directions to the inlet valve axes and the effects of port deactivation, throttling and exhaust gas recirculation were examined. The implications of the results for intake port design are discussed.
    Additional Material: 13 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Archives of Insect Biochemistry and Physiology 1 (1983), S. 1-15 
    ISSN: 0739-4462
    Keywords: Stomoxys calcitrans ; mannosyl transferase ; stable fly ; glycosyl transferase ; dolicholphosphate ; Chemistry ; Food Science, Agricultural, Medicinal and Pharmaceutical Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Particulate fractions (10,000g) from pupae of Stomoxys calcitrans transfer [14C]-mannose from GDP-[14C]-mannose to dolichol monophosphate and proteins. Production of the mannosyl lipid was inhibited by Mn2+, UDP, GMP, GDP, and EDTA. The insect growth regulator diflubenzuron had no effect on mannosyl transferase activity. Dolichol monophosphate and Mg2+ stimulated mannosyl transferase activity. The mannosyl lipid product was identified as mannosyl-phosphoryl-dolichol (Man-P-Dol). The apparent Km and Vmax values for the formation of Man-P-Dol using GDP-[14C]-Man while holding dolichol phosphate constant were 2.4 ± 0.9 μM and 9.4 ± 2.3 pmol Man-P-Dol·min-1·mg-1 protein, respectively. The apparent Km and Vmax values using dólichol phosphate while holding GDP-Man constant were 2.2 ± 1.2 μM and 18.5 ± 1.7 pmol Man-P-Dol·min-1·mg-1 protein.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
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