ISSN:
1573-7284
Keywords:
Transformation
;
Streptococcus pneumoniae
;
Tetracycline resistance
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Transformation of pneumococcus was used to detect homology among tetracycline resistance determinants of clinical isolates of Streptococcus pneumoniae. A strain of pneumococcus containing a mutated tet determinant (tet-3), of class M, integrated into the chromosome was used as a recipient in transformation experiments, where donor DNA was from the tetracycline resistant isolates. 34/34 strains appeared to have tet determinants homologous to tet-3 (i.e. tet M). Still using transformation it was possible to determine that the tet-3 transforming activity of DNA from Tn916 and S. pneumoniae BM6001 was contained in a 5 kb HincII fragment. For this purpose a transformation technique where donor DNA was directly taken from low melting point agarose gels was standardized and used.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00157016
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