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  • 1
    Electronic Resource
    Electronic Resource
    Rapid redox equilibrium between the mitochondrial Q pool and cytochrome b during triphasic reduction of cytochrome b by succinate
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Bioenergetics 851 (1986), S. 457-468 
    Details
    ISSN: 0005-2728
    Keywords: (Pig-heart mitochondria) ; Cytochrome b ; Redox equilibrium ; Respiratory chain ; Succinate:cytochrome c reductase ; Ubiquinone pool
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0005-2728(86)90082-4
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  • 2
    Electronic Resource
    Electronic Resource
    The effect of ring substituents on the mechanism of interaction of exogenous quinones with the mitochondrial respiratory chain
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Bioenergetics 851 (1986), S. 469-474 
    Details
    ISSN: 0005-2728
    Keywords: (Pig-heart mitochondria) ; Cytochrome bc"1 complex ; Plastoquinone ; Respiratory chain ; Succinate:cytochrome c reductase ; Ubiquinone
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0005-2728(86)90083-6
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  • 3
    Electronic Resource
    Electronic Resource
    High-density perfusion culture of insect cells with a BioSep ultrasonic filter (1998)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 59 (1998), S. 351-359 
    Details
    ISSN: 0006-3592
    Keywords: bioreactor ; high density ; insect cells ; perfusion ; Sf9 ; ultrasonic filter ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The baculovirus/insect cell expression system has provided a vital tool to produce a high level of active proteins for many applications. We have developed a very high-density insect cell perfusion process with an ultrasonic filter as a cell retention device. The separation efficiency of the filter was studied under various operating conditions. A cell density of over 30 million cells/mL was achieved in a controlled perfusion bioreactor and cell viability remained greater than 90%. Sf9 cells from a high-density culture and a spinner culture were infected with two recombinant baculoviruses expressing genes for the production of human chitinase and monocyte-colony inhibition factor. The protein yield on a cell basis from infecting high-density Sf9 cells was the same as or higher than that from the spinner Sf9 culture. Virus production from the high-density culture was similar to that from the spinner culture. The results show that the ultrasonic filter did not affect insect cells' ability to support protein expression and virus production following infection with baculovirus. The potential applications of the high-density perfusion culture for large-scale protein expression from Sf9 cells are also highlighted. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 59:351-359, 1998.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
  • 4
    Electronic Resource
    Electronic Resource
    The correlation between phase behavior and structure of a four-component system, poly-ethylene glycol/sodium dodecyl sulfate/pentanol/H2OThis paper was presented at the 5th International Conference on Surface and Colloid Science and the 59th Division of Colloid and Interface Science, American Chemical Society, held in Potsdam, New York, June, 1985. (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 32 (1986), S. 4383-4392 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The change in the concentration of sodium dodecyl sulfate (SDS) can, in fact, affect directly the correlation between phase behavior and structure of a four-component system, polyethylene glycol/sodium dodecyl sulfate/pentanol/H2O. Experimental results indicate that the phenomena of microemulsion and lamellar liquid crystals can take place at a concentration of SDS greater than 5% and 17% by weight, respectively. In our experiments, these liquid crystals are tested and found to be hydrophilic and their rheological properties to be non-Newtonian as well.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1986.070320408
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  • 5
    Electronic Resource
    Electronic Resource
    Growth models of cultures with two liquid phases: IV. Cell adsorption, drop size distribution, and batch growth (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 12 (1970), S. 713-746 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: In this work, a mathematical model which can be used to describe butch growth in fermentations with two liquid phases present is developed for systems in which the growth limiting substrate is dissolved in the dispersed phase. The model takes into account the drop size distribution, the rate of adsorption of cells on the drop surface, the rate of desorption of cells from the drop surface, substrate transport between phases, phase equilibrium, and growth kinetics. The model also considers the effect, of coalescence and redispersion of oil drops in the system. It is assumed that the composition of the dispersed phase is such that substrate utilization from it causes little or no change in the interfacial area. A discrete uniform distribution and a discrete normal distribution which is obtained from an experimental distribution curve are used as drop size distributions. Simulation results are obtained for a wide range of parameter values using the IBM S/360 Continuous System Modeling Program.
    Additional Material: 23 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260120505
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  • 6
    Electronic Resource
    Electronic Resource
    Recovery of canola meal proteins by precipitation (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 40 (1992), S. 63-68 
    Details
    ISSN: 0006-3592
    Keywords: rapeseed protein recovery ; isoelectric precipitation ; HMP ; CMC ; ammonium sulphate ; nitrogen yield ; aggregate size ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Recovery of rapeseed proteins from defatted canola meal by precipitation was investigated. The ability of different precipitating agents, such as sodium hexametaphosphate (HMP), carboxymethylcellulose (CMC), ammonium sulphate, and isoelectric precipitation using HCl, were evaluated based on the yield and mean size of protein aggregates. Almost 94% of dissolved protein was precipitated in the presence of 2.7M ammonium sulphate, while the largest mean protein particle size (32 μm) was obtained in the presence of HMP at pH 3.3.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260400110
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  • 7
    Electronic Resource
    Electronic Resource
    Hydrolysis of carbonyl sulfide in a gas-liquid reactor (1988)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 34 (1988), S. 158-162 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690340122
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  • 8
    Electronic Resource
    Electronic Resource
    A novel radioactive particle tracking facility for measurement of solids motion in gas fluidized beds (1985)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 31 (1985), S. 465-473 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Measurements of solids motion in a fluidized bed have been made in a computer-aided particle tracking facility. A radioactive tracer particle, dynamically identical to the solid particles to be studied, was mixed with the solids in the bed. The gamma radiation from the tracer was continuously monitored by a large number of scintillation detectors located around the bed, providing information on the tracer's instantaneous location. Prudent use was made of the purposely introduced redundant distance data to achieve improved accuracy. The recognition of the existence of secondary emission due to the interaction of the primary radiation and other system materials and the subsequent devising of a scheme to mitigate its effect contribute much to the success. Results for a bed with a uniform air distributor plate show the existence of two counter-rotating toroidal vortices whose relative sizes and strengths vary with the fluidizing velocity. Fluctuating motions at low frequencies ranging from 1.6 to 16 Hz have been observed at various locations in the bed.
    Additional Material: 13 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690310314
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  • 9
    Electronic Resource
    Electronic Resource
    The effect of eccentricity on the terminal velocity of the cylinder in a falling cylinder viscometer (1968)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 14 (1968), S. 123-127 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The theoretical equations for the flow of Newtonian fluids in a falling cylinder viscometer with eccentricity are developed. The error in fall velocity of the cylinder caused by eccentricity is determined and presented in tabular form. An approximate method to determine the error in fall velocity caused by eccentricity for non-Newtonian fluids of the power law type is also presented. For the Newtonian case, n=1, the approximate method is shown to give excellent agreement with the exact method.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690140122
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  • 10
    Electronic Resource
    Electronic Resource
    Analysis of entrance and exit effects in a falling cylinder viscometer (1972)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 18 (1972), S. 146-149 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A variational method has been used to study the entrance and exit effects in a falling cylinder viscometer for creeping and noncreeping flow. The computed result indicate solution convergence for NRe less than 100 and agree with experiment within ± 0.5% for NRe less than 25.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690180127
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