ISSN:
1432-2242
Keywords:
Sugar beet
;
Cytoplasmic male sterility
;
Mitochondrial and chloroplast DNAs
;
Restriction endonuclease fragment analysis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Mitochondrial (mt) and chloroplast (ct) DNAs from sugar beet lines carrying normal and introduced sources of male sterile cytoplasms have been characterized and compared on the basis of restriction enzyme analysis. Normal cytoplasm was shown to contain mt and ctDNAs which differed from those of the male sterile cytoplasms examined in the present investigation. On the other hand, four groups of male sterile cytoplasms could be differentiated by their own characteristic mtDNA digest patterns, while two were separated by ctDNA comparisons. In addition, a greater degree of variability of the mitochondrial genome is suggested. Our results also imply strict maternal inheritance of mt and ctDNAs. Thus, the organelle DNA assay provides a positive and alternative means of identifying various male sterile cytoplasms.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00252051
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