ISSN:
1432-203X
Keywords:
Key wordsMatteuccia struthiopteris
;
Ostrich fern
;
Suspension culture
;
Shoot primordia
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A rapid and efficient in vitro plant regeneration method was developed for Matteuccia struthiopteris (L.) Todaro (Ostrich fern). Side shoots, originating in meristems of sectioned rhizomes, were used as explant material. A very high rate of meristem multiplication was achieved by culturing the explants in half-strength MS liquid medium supplemented with 2.0 mg/l N-(4-Pyridyl)-N′-phenylurea (4-PU) and 0.5 mg/l thidiazuron (TDZ). Multiplication of the shoot primordia was faster in suspension culture than on solid medium. Rhizogenesis and growth of regenerants were best achieved on hormone-free one-quarter-strength MS solid medium amended with 0.4% agar and 1.0% activated charcoal. Regenerated plantlets continued to grow after transfer to soil in a phytotron.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050557
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