ZIB

Treffer pro Seite

Treffer 1 - 1 | 1 Treffer

Sortierung

Digitale Medien

High level secretion of TaqI restriction endonuclease by recombinant Escherichia coli (1999)

Toksoy, E. ; Özdinler, P.H. ; Önsan, Z.İ. ; [weitere]

Springer

Biotechnology techniques 13 (1999), S. 803-808

zur Merkliste hinzufügen auf der Merkliste

Details

ISSN: 1573-6784

Schlagwort(e): TaqI restriction endonuclease ; MBP fusion protein ; secretion ; extracellular production

Quelle: Springer Online Journal Archives 1860-2000

Thema: Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: Abstract The production and high level secretion of TaqI restriction endonuclease using bacterial secretion signal within the malE gene was achieved by cloning the PCR-amplified gene from Thermus aquaticus into E. coli. The maltose binding protein (MBP) part of the MBP-TaqI fusion protein expressed by this construct did not interfere with the biological activity of the TaqI restriction endonuclease. E. coli XL1 carrying pH185 produced 332 U ml−1 TaqI endonuclease 81% of which was secreted into the medium without apparent cell lysis. Optimization of culture conditions and selection of the host strain were found to be important for the efficient extracellular production of this protein.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1023/A:1008938302312

Permalink

Bibliothek	Standort	Signatur	Band/Heft/Jahr	Verfügbarkeit

Andere fanden auch interessant ...

Artikel (Nationallizenzen)

Volltext

Treffer 1 - 1 | 1 Treffer