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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell reports 14 (1995), S. 571-574 
    ISSN: 1432-203X
    Keywords: Crassostrea gigas ; Dictyopteris prolifera ; Marine brown alga ; Protoplast ; Single cell
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Protoplasts were isolated from thalli of Dictyopteris prolifera using a mixture of crude enzymes from vicera of live oysters (Crassostrea gigas) and the following commercial enzymes: an abalone enzyme, cellulase, polygalacturonase and hemicellulase. The enzyme mixtures produced up to 3.3 × 107 cells per l g of tissue fresh weight. The conversion to protoplasts of the cells was about 100% using the oyster enzyme or the abalone enzyme alone. The optimum pH for protoplast isolation was 6.0 and 20 hours were required for conversion to protoplasts.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1084
    Keywords: Digital radiography ; Image processing ; Subtraction ; Thorax ; Tomography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Digital tomosynthesis makes it possible to reconstruct multiple tomographs from digital data obtained during a single tomographic motion and permits digital processing, which adds a number of special advantages to the well-known advantages of conventional tomography. We performed digital tomosynthesis with a fluororadiographic TV unit with tomographic function which was capable of producing pulsed low-and high-energy X-rays alternately, and we studied digital image processing to improve the image clarity of the reconstructed tomographs. To identify the optimal parameters for processing image data by means of spatial frequency filtration we evaluated the spatial frequency distribution of image data in linear tomographs of the lung, and on the basis of the results of this study we developed several types of digital image processing to reduce tomographic blur and system noise, to improve visualisation of faint opacities, to reduce resistant tomographic blur as well as overall blur, and to generate low-noise bone images based on dual-energy subtraction tomosynthesis.
    Type of Medium: Electronic Resource
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