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  • 1
    ISSN: 1573-6830
    Keywords: Torpedo marmorata ; rat ; ox ; frog ; synaptic vesicles ; antiserum ; retina ; cholinergic nerve terminals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary 1. An antiserum to cholinergic synaptic vesicles fromTorpedo marmorata which recognizes the heparan-like glycosaminoglycan present in these vesicles and reacts specifically with peripheral and presumed central cholinergic nerve terminals in mammalian species has been tested with retinas from a variety of species. 2. The antiserum recognizes specific sites in the inner and outer plexiform layers of retinas of rat, ox and frog. 3. The distribution is similar to that reported forα-bungarotoxin, a reagent specific for acetylcholine receptors. 4. Immunoreactive material was absent from retinal cell bodies. 5. Its appearance in development in the two layers coincided with the appearance of synapses in these layers. 6. We conclude that the antigenic sites responding to the anti-vesicle anti-serum are associated with the cholinergic endings in the retina.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 218 (1981), S. 355-373 
    ISSN: 1432-0878
    Keywords: Cholinergic nerve terminals ; Presynaptic plasma membrane ; Indirect immunofluorescence histochemistry ; Torpedo marmorata ; Mammals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Most of the published light-microscopic methods for the localization of cholinergic nerve pathways present various difficulties of interpretation. The production and characterization of an antiserum that binds specifically to cholinergic terminals is described. The antiserum was raised to small synaptosomes prepared from the purely cholinergic electric organ of Torpedo marmorata. It was shown to lyse cholinergic synaptosomes in a mixed population derived from guinea-pig cortex. After partial purification by adsorption onto nonspecific antigens, it was used to label nerve endings in several tissues of Torpedo, rats and guinea pigs using indirect immunofluorescence histochemistry. The antiserum appears to provide a highly specific means of localizing cholinergic nerve endings in these tissues.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-0878
    Keywords: Cholinergic vesicle antigen ; Axonal transport ; Exo/endocytosis ; Indirect immunofluorescence histochemistry ; Torpedo marmorata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary An antiserum against a specific component (a glycosamino glycan) of the cholinergic synaptic-vesicle of Torpedo marmorata has been used to investigate the localization of the component in the cell body, its movement within the electromotor axon and its fate within the nerve terminal upon electrical stimulation. After immunofluorescent staining, spots are observed throughout the cytoplasm of the lobe perikarya, although they are concentrated in the region of the axon hillock. Ligation of the electromotor nerves leading from the lobe to electric organ produces a proximal build-up of material which stains readily with the antivesicle antiserum, indicating that the vesicle antigen is transported from the cell body to the nerve terminal. A marked increase in indirect immunofluorescent staining of the electric organ is observed in the nerve ending upon electrical stimulation. We interpret this result as fusion of the vesicles with the presynaptic plasma membrane and exteriorization of the vesicle antigen to the extracellular space, thereby facilitating its staining. After recovery of the system the fluorescence declines, a result that is consistent with the reinternalization of the vesicle antigen into the core of reformed vesicles. The results support a mechanism whereby vesicles recycle within the nerve terminal and transmitter is released by exocytosis.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-0878
    Keywords: Vesicle recycling ; Immunohistochemistry ; Glycosaminoglycan ; Electrical stimulation ; Torpedo marmorata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Semiquantitative immunohistochemical methods were used to demonstrate that at least some of the glycosaminoglycan contained within cholinergic synaptic vesicles is recycled during successive electrical stimulations of the electric organ of Torpedo marmorata.
    Type of Medium: Electronic Resource
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