ISSN:
1617-4623
Keywords:
RNA polymerase
;
Promoters
;
Bacillus subtilis
;
Sigma
;
Transcription termination
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Sequencing data indicated that the RNA polymerase (σ43) operon of Bacillus subtilis consisted of three genes, P23 (function unknown), dnaE (DNA primase), and rpoD (σ43) (Wang and Doi 1986a). S1 nuclease mapping experiments with RNA from various stages of growth demonstrated the presence of two overlapping σ43 promoters that controlled the expression of the operon during growth and a σ37 promoter that regulated the expression of the operon during the sporulation phase. This promoter switching mechanism ensured that this important operon would be expressed during different nutritional states of the cell and also illustrated a function for the minor RNA polymerase σ37 holoenzyme in the expression of genes which are normally expressed during the logarithmic phase of growth. The location of the transcription termination signal confirmed that the σ43 operon consists of three genes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00331498
Permalink
