ISSN:
1615-6102
Keywords:
In vitro shoots
;
Pisum sativum
;
Meristematic cells
;
Shoot apices
;
Transformation
;
Agrobacterium
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Different tissues in cultured pea shoots were inoculated withAgrobacterium tumefaciens wild types C 58 and ACH 5 andA. rhizogenes wild type 9402. The C 58 and 9402 bacteria induced the formation of tumours and hairy roots respectively while the ACH 5 was inactive. The younger the tissue the more rapidly it responded to the active bacteria. The shoot apex was the most reactive organ and developed into a tumour, theA. rhizogenes tumours subsequently giving rise to transformed hairy roots. Histological examination showed that transformed cells (including those within the apical dome) initially became highly vacuolate before dividing rapidly to form a tumour. These changes were accompanied by cell division in surrounding tissues.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02079328
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