ISSN:
1432-203X
Keywords:
Key words Fungal chitinase
;
Transgenic tobacco
;
Antifungal activity
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We have studied whether a chitinase involved in cell autolysis of a filamentous fungus, Rhizopus oligosporus, can operate as an antifungal defense system in tobacco. The chi1 gene was introduced into tobacco by the Agrobacterium tumefaciens leaf disc system. Among 22 transgenic tobacco plants, 2 were selected and their individual homozygous progeny, Tch1-1 and Tch2-1, were studied. Chitinase activity in the extracts of young leaves from Tch1-1 or Tch2-1, in which the chi1 gene product was detected by Western blot analysis, was three- to four-fold higher than that from the control plants. A fungal infection assay on the leaves infected with the discomycete pathogens Sclerotinia sclerotiorum and Botrytis cinerea revealed that the symptoms observed with these two were remarkably suppressed as compared with the control leaves.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01092762
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