ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Amino silica gel
;
Neuraminic acids
;
UV detection at nanomole level
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary Neuraminic acid derivatives were separated easily by liquid chromatography on amino phases and using acetonitrile/15 mM phosphate buffer, pH 5.2 as mobile phase. Detection at 200 nm allowed determination of substances in the nanomole range. All solvent systems were used isocratically at room temperature. The separation did not depend on ion-exchange but on a partition mechanism, the solutes being separated according to their polarity. Thus, it was possible to optimize the resolution by varying the stationary phase and the composition of the eluent.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02259693
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