ISSN:
1432-069X
Keywords:
Electron spin resonance
;
Ultraviolet light
;
B-16 Melanoma cells
;
Membrane fluidity
;
Phospholipase A2
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Electron spin resonance spectroscopy using the spin probe (5-, 12-and 16-deoxylstearic acid) was employed to analyze the changes in membrane fluidity in B-16 melanoma cells following UV-B exposure. The UV exposure resulted in the immediate accumulation of lipid peroxide, being accompanied by a change in membrane fluidity. The 12-DSA is the most sensitive to the changes in membrane organization caused by UV light. Na+,K+-ATPase activity was regulated by a change in membrane fluidity. Following UV exposure, the release of the prelabeled arachidonic acid from the cells was observed immediately. Ca2+-dependent calmodulin-dependent phospolipase A2-like activity was involved in the UV-stimulated arachidonic acid release from phospholipid.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00427661
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