ISSN:
1040-0397
Keywords:
Field-effect transistor
;
ISFET
;
Urease
;
NAD+
;
Dehydrogenase
;
Buffer capacity
;
Redox-FET
;
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
A specially designed field-effect transistor (FET) with a significantly enlarged gate area was applied in a classical urea enzyme FET (ENFET). The resulting high stability and sensitivity toward pH shifts make it predestinated for the measurement of H+ produced in the equilibrium of NAD+ -dependent enzymatic reactions, especially when the equilibrium is shifted by a subsequent reaction. As a model, the glucose dehydrogenase (GDH) reaction connected to an ion-sensitive field-effect transistor (ISFET) is demonstrated by which glucose could be determined in the range from 1 to 40 mM. A platinum electrode on the gate of the FET permits the measurement of reduction equivalents (NADH) by means of the recently, reported chronopotentiometrical methods. Thus, in principle, a way toward a redox ENFET is shown.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elan.1140061106
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