ISSN:
1432-2242
Keywords:
Zea mays
;
Parentage determination
;
Varietal identification
;
Genetic polymorphisms
;
Genetic markers
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Using a novel procedure based on the polymerase chain reaction, we have developed a rapid, efficient, and economical method for identifying plant genotypes. The arbitrarily primed polymerase chain reaction (AP-PCR) generates reproducible fingerprints from any organism, without the need for DNA sequence information. These fingerprints include DNA fragment polymorphisms that can be (1) used for varietal identification and parentage determination, (2) followed in segregating populations produced by crosses, (3) used as markers for the construction of genetic maps, and (4) used to generate dendograms of phylogenetic relationships, especially at the intraspecific level. AP-PCR requires only minute quantities of DNA (10–25 ng per reaction) and therefore can be used in situations in which DNA is limiting. We demonstrate the use of AP-PCR to identify inbred parents of hybrid maize plants in double-blind experiments.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00588601
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