ISSN:
1432-2242
Keywords:
Key wordsMalus×domestica
;
Venturia inaequalis
;
Malus floribunda
;
Genetic map
;
Disease resistance
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A detailed genetic map has been constructed in apple (Malus×domestica Borkh.) in the region of the V f gene. This gene confers resistance to the apple scab fungus Venturia inaequalis (Cooke) Wint‥ Linkage data on four RAPD (random amplified polymorphic DNA) markers and the isoenzyme marker PGM-1, previously reported to be linked to the V f gene, are integrated using two populations segregating for resistance to apple scab. Two new RAPD markers linked to V f (identified by bulked segregant analysis) and a third marker previously reported as being present in several cultivars containing V f are also placed on the map. The map around V f now contains eight genetic markers spread over approximately 28 cM, with markers on both sides of the resistance gene. The study indicates that RAPD markers in the region of crab apple DNA introgressed with resistance are often transportable between apple clones carrying resistance from the same source. Analysis of co-segregation of the resistance classes 3A (weakly resistant) and 3B (weakly susceptible) with the linked set of genetic markers demonstrates that progeny of both classes carry the resistance gene.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s001220050306
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