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  • 1
    Electronic Resource
    Electronic Resource
    Death rate in a small air-lift loop reactor of vero cells grown on solid microcarriers and in macroporous microcarriers (1996)
    Springer
    Cytotechnology 21 (1996), S. 45-59 
    Details
    ISSN: 1573-0778
    Keywords: air lift ; macroporous ; microcarrier ; shear ; sparging ; Vero cell
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The death rate of Vero cells grown on Cytodex-3 microcarriers was studied as a function of the gas flow rate in a small air-lift loop reactor. The death rate may be described by first-order death-rate kinetics. The first-order death-rate constant as calculated from the decrease in viable cells, the increase in dead cells and the increase in LDH activity is linear proportional to the gas flow rate, with a specific hypothetical killing volume in which all cells are killed of about 2·10−3 m3 liquid per m3 of air bubbles. In addition, an experiment was conducted in the same air-lift reactor with Vero cells grown inside porous Asahi microcarriers. The specific hypothetical killing volume calculated from this experiment has a value of 3·10−4 m3 liquid per m3 of air bubbles, which shows that the porous microcarriers were at least in part able to protect the cells against the detrimental hydrodynamic forces generated by the bubbles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00364836
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Death rate in a small air-lift loop reactor of vero cells grown on solid microcarriers and in macroporous microcarriers (1997)
    Springer
    Cytotechnology 23 (1997), S. 61-75 
    Details
    ISSN: 1573-0778
    Keywords: air lift ; macroporous ; microcarrier ; shear ; sparging ; Vero cell
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The death rate of Vero cells grown on Cytodex-3 microcarrierswas studied as a function of the gas flow rate in a smallair-lift loop reactor. The death rate may be described byfirst-order death-rate kinetics. The first-order death-rateconstant as calculated from the decrease in viable cells, theincrease in dead cells and the increase in LDH activity islinear proportional to the gas flow rate, with a specifichypothetical killing volume in which all cells are killed ofabout 2.10-3m3 liquid per m3 of air bubbles.In addition, an experiment was conducted in the sameair-lift reactor with Vero cells grown inside porous Asahimicrocarriers. The specific hypothetical killing volumecalculated from this experiment has a value of 3.10-4m3 liquid per m3 of air bubbles, which shows thatthe porous microcarriers were at least in part able to protectthe cells against the detrimental hydrodynamic forcesgenerated by the bubbles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/B:CYTO.0000010399.23373.65
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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