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  • 1
    Electronic Resource
    Electronic Resource
    Application of two microsatellite sequences in wheat storage proteins as molecular markers (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 247-252 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Microsatellite markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In eukaryotes, tandem arrays of simple-sequence repeat sequences can find applications as highly variable and multi-allelic PCR-based genetic markers. In hexaploid bread wheat, a large-genome inbreeding species with low levels of RFLP, di- and trinucleotide tandem repeats were found in 22 published gene sequences, two of which were converted to PCR-based markers. These were shown to be genome-specific and displayed high levels of variation. These characteristics make them especially suitable for intervarietal breeding applications.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222209
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Low levels of DNA sequence variation among adapted genotypes of hexaploid wheat (1999)
    Springer
    Theoretical and applied genetics 99 (1999), S. 192-198 
    Details
    ISSN: 1432-2242
    Keywords: Key words PCR markers ; Sequence-tagged-site ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  PCR products from regions corresponding to sequences hybridising to wheat RFLP probes were sequenced in order to establish the level of DNA sequence variation among adapted wheat genotypes. Hexaploid bread wheat shows a very low rate of nucleotide polymorphism, approximately 1 polymorphic nucleotide per 1000 basepairs. Differences in PCR product length can be exploited to design genome-specific amplicons, which may have use in gene tagging or in diagnostic applications. Interpretation of results may be complicated by the simultaneous amplification of orthologous and paralogous sequences. These findings have significant implications for the use of STS markers in wheat and other polyploid species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051224
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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