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  • 1
    Electronic Resource
    Electronic Resource
    The use of fluorescent in situ hybridization for detection of the t(2;5)(p23;q35) translocation in anaplastic large-cell lymphoma (1997)
    Springer
    Annals of oncology 8 (1997), S. 65-69 
    Details
    ISSN: 1569-8041
    Keywords: anaplastic large-cell lymphoma ; fluorescent in situ hybridization ; Hodgkin's disease ; Ki-1 lymphoma ; PCR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background: Anaplastic large-cell lymphoma (ALCL) is a recently recognized, distinctive type of non-Hodgkin's lymphoma characterized by anaplastic large-cell cytology and expression of a member of the TNF-receptor family CD30. A characteristic chromosomal translocation has been identified in ALCL of T- or null-cell lineage which juxtaposes a novel tyrosine kinase (anaplastic lymphoma kinase, ALK) located at 2p23 with the nucleophosmin gene (NPM) at 5q35. A chimeric mRNA transcript is produced, and the translocation results in constitutive expression of a truncated form of the ALK protein, p80. There is controversy concerning whether or not the translocation occurs in Hodgkin's disease. The aim of this study was to develop a methodology for fluorescent in situ hybridization (FISH) to detect the t(2;5)(p23;q35), and to compare the results with conventional cytogenetics, reverse-transcriptase PCR and immunostaining for the p80protein. Patients and methods: Twenty-five cases of malignant lymphoma (11 ALCL and 14 HD) were studied. Immunohistochemistry was performed to confirm the diagnosis and for analysis of p80 expression. Conventional cytogenetics were analyzed on G-banded metaphase spreads. FISH was performed using whole chromosome paints for chromosomes 2 and 5 on metaphase spreads and YAC probes for inter phase nuclei. Reverse-transcriptase PCR using primers for ALK and NPM was used to amplify the translocation breakpoint in extracted mRNA. Results: Among 11 cases of ALCL examined by FISH, the translocation was detected in 4. Two of these cases also had RT-PCR and p80 staining performed, with positive results. Among 7 cases where the t(2;5) was not detected by FISH, 3 cases were examined by RT-PCR with negative results and4 cases by p80 staining, also negative. The RT-PCR was negative in all 14cases of Hodgkin's disease, 4 of which were also examined by FISH and found to be negative. Conclusion: Fluorescent in situ hybridization is useful method for detection of the t(2;5)(p23;q35) in anaplastic large-cell lymphoma. The results concur with those of RT-PCR for the chimeric transcript and immunostaining for the p80 protein. The frequency with which the translocation was found was 36% in this small series, and no evidence of the translocation was found in cases of Hodgkin's disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008234301024
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    The use of fluorescent in situ hybridization for detection of the t(2;5)(p23;q35) translocation in anaplastic large-cell lymphoma (1997)
    Springer
    Annals of oncology 8 (1997), S. 65-69 
    Details
    ISSN: 1569-8041
    Keywords: anaplastic large-cell lymphoma ; fluorescent in situ hybridization ; Hodgkin's disease ; Ki-1 lymphoma ; PCR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background: Anaplastic large-cell lymphoma (ALCL) is a recentlyrecognized, distinctive type of non-Hodgkin's lymphoma characterized byanaplastic large-cell cytology and expression of a member of theTNF-receptor family CD30. A characteristic chromosomal translocation hasbeen identified in ALCL of T- or null-cell lineage which juxtaposes a noveltyrosine kinase (anaplastic lymphoma kinase, ALK) located at 2p23 with thenucleophosmin gene (NPM) at 5q35. A chimeric mRNA transcript is produced,and the translocation results in constitutive expression of a truncated formof the ALK protein, p80. There is controversy concerning whether or not thetranslocation occurs in Hodgkin's disease. The aim of this study was todevelop a methodology for fluorescent in situ hybridization (FISH) to detectthe t(2;5)(p23;q35), and to compare the results with conventionalcytogenetics, reverse-transcriptase PCR and immunostaining for the p80protein. Patients and methods: Twenty-five cases of malignant lymphoma (11 ALCLand 14 HD) were studied. Immunohistochemistry was performed to confirm thediagnosis and for analysis of p80 expression. Conventional cytogenetics wereanalyzed on G-banded metaphase spreads. FISH was performed using wholechromosome paints for chromosomes 2 and 5 on metaphase spreads and YACprobes for interphase nuclei. Reverse-transcriptase PCR using primers forALK and NPM was used to amplify the translocation breakpoint in extractedmRNA. Results: Among 11 cases of ALCL examined by FISH, the translocation wasdetected in 4. Two of these cases also had RT-PCR and p80 stainingperformed, with positive results. Among 7 cases where the t(2;5) was notdetected by FISH, 3 cases were examined by RT-PCR with negative results and4 cases by p80 staining, also negative. The RT-PCR was negative in all 14cases of Hodgkin's disease, 4 of which were also examined by FISH and foundto be negative. Conclusion: Fluorescent in situ hybridization is useful methodfor detection of the t(2;5)(p23;q35) in anaplastic large-cell lymphoma. Theresults concur with those of RT-PCR for the chimeric transcript andimmunostaining for the p80 protein. The frequency with which the translocationwas found was 36% in this small series, and no evidence of thetranslocation was found in cases of Hodgkin's disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008234301024
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Clinical evaluation of the partition model for estimating radiation doses from yttrium-90 microspheres in the treatment of hepatic cancer (1997)
    Springer
    European journal of nuclear medicine 24 (1997), S. 293-298 
    Details
    ISSN: 1619-7089
    Keywords: Partition model ; Clinical evaluation ; Yttrium-90 microspheres ; Hepatic cancer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Radiation doses to the tumour and non-tumorous liver compartments from yttrium-90 microspheres in the treatment of hepatic cancer, as estimated by a partition model, have been verified by correlation with the actual doses measured with a beta probe at open surgery. The validity of the doses to the lungs, the tumour and non-tumorous liver compartment as estimated by the partition model was further evaluated in clinical settings. On the basis of the observation that one of three patients who received more than 30 Gy from a single treatment and one of two patients who received more than 50 Gy from multiple treatments developed radiation pneumonitis, it was deduced that an estimated lung dose 〈30 Gy from a single treatment and a cumulative lung dose 〈50 Gy from multiple treatments were probably the tolerance limits of the lungs. Three of five patients who received lung doses 〉30 Gy as estimated by the partition model and were predicted to develop radiation pneumonitis, did so despite the use of partial hepatic embolization to reduce the degree of lung shunting. Furthermore, a higher radiological response rate and prolonged survival were found in the group of patients who received higher tumour doses, as estimated by the partition model, than in the group with lower estimated tumour doses. Thus the radiation doses estimated by the partition model can be used to predict (a) complication rate, (b) response rate and (c) duration of survival in the same manner as the actual radiation doses measured with a beta probe at open surgery. The partition model has made selective internal radiation therapy using90Y microspheres safe and repeatable without laparotomy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01728766
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Clinical evaluation of the partition model for estimating radiation doses from yttrium-90 microspheres in the treatment of hepatic cancer (1997)
    Springer
    European journal of nuclear medicine 24 (1997), S. 293-298 
    Details
    ISSN: 1619-7089
    Keywords: Key words: Partition model ; Clinical evaluation ; Yttrium-90 microspheres ; Hepatic cancer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Radiation doses to the tumour and non-tumorous liver compartments from yttrium-90 microspheres in the treatment of hepatic cancer, as estimated by a partition model, have been verified by correlation with the actual doses measured with a beta probe at open surgery. The validity of the doses to the lungs, the tumour and non-tumorous liver compartment as estimated by the partition model was further evaluated in clinical settings. On the basis of the observation that one of three patients who received more than 30 Gy from a single treatment and one of two patients who received more than 50 Gy from multiple treatments developed radiation pneumonitis, it was deduced that an estimated lung dose 〈30 Gy from a single treatment and a cumulative lung dose 〈50 Gy from multiple treatments were probably the tolerance limits of the lungs. Three of five patients who received lung doses 〉30 Gy as estimated by the partition model and were predicted to develop radiation pneumonitis, did so despite the use of partial hepatic embolization to reduce the degree of lung shunting. Furthermore, a higher radiological response rate and prolonged survival were found in the group of patients who received higher tumour doses, as estimated by the partition model, than in the group with lower estimated tumour doses. Thus the radiation doses estimated by the partition model can be used to predict (a) complication rate, (b) response rate and (c) duration of survival in the same manner as the actual radiation doses measured with a beta probe at open surgery. The partition model has made selective internal radiation therapy using 90Y microspheres safe and repeatable without laparotomy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01728766
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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