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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 87 (1993), S. 33-37 
    ISSN: 1432-2242
    Keywords: Zein ; Zea mays L. ; Corn ; Genetic linkages ; RP-HPLC ; Proteins ; Prolamins ; Inheritance ; Quantitation ; Model
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A genetic model developed by Bogyo et al. (1988) for quantitatively inherited triploid endosperm characters (an extension of the well-known Mather-Jinks model) is not well-suited for estimating epistatic interaction effects because it requires the assumption that, in segregating loci, all alleles positively affecting a particular character are in one of the inbred parental lines. To better explain zein inheritance in maize, a new model was developed not relying on this assumption. This model was tested by quantitative analysis of A/B zeins, the predominant prolamin storage proteins of maize, using reversed phase high-performance liquid chromatography of two inbred lines, their reciprocal F1 crosses, the F2 generation, backcrosses, and reciprocal backcrosses to both parent lines. The model required epistatic components to be included for an excellent fit for most protein peaks.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5060
    Keywords: Zea mays ; maize ; hybrid ; inbred line ; pedigree determination ; electrophoresis ; chromatography ; RP-HPLC
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Determination of pedigree by laboratory means can provide an initial and rapid check of hybrid identity in studies designed to compare genotypes. In order to test the accuracy of such determinations, isozymic and chromatographic data were used in an attempt to ascribe pedigrees to 25 hybrids that were identified only by a letter code from a list of 30 inbred lines. The pedigrees of 9 single-cross hybrids were correctly deduced as was the direction in which each cross was made. The pedigrees of 2 hybrids could only be determined ambiguously, however these hybrids were reciprocals and the biochemical data were unable to distinguish between 2 related candidate inbred lines. Pedigrees of 3 hybrids were either incompletely determined or could not be determined from the list of 30 candidate inbred lines and were thus correctly identified as non-Pioneer® brand hybrids. Pedigrees of 9 3-way hybrids could be determined accurately but the pedigrees of 2 non-Pioneer® brand hybrids could not be deduced completely. The ability to correctly ascribe pedigrees of hybrids shows that these data provide a rapid means of determining whether additional field and laboratory tests would be warranted in tests designed to compare genotypic similarity of hybrids.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-5060
    Keywords: Cultivar identification ; electrophoresis ; isoenzyme ; minimum distance ; reversed-phase-HPLC ; Zea mays L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Zein chromatographic and isozyme electrophoretic data were used to characterize 61 hybrids of maize (Zea mays L.) that are of current widespread usage in France. A similar study was also carried out for 88 hybrids that are widely available in the central corn belt of the U.S. Objectives were to 1) investigate the degree to which isozyme and chromatographic data could provide unique hybrid identification; 2) to evaluate the wealth of genetic diversity available among hybrids that are currently cultivated in France and in the U.S.; and 3) to compare the patterns and extent of genetic diversity found in both countries with respect to their registration, breeding, and seed production practices. Isozyme data showed that all French hybrids were unique germplasm. However, numerous hybrids had chromatographic profiles that were identical for 11 major peaks and these hybrids could thus have some common germplasm at least among their female parental lines. Although there was a broad base of isozymic diversity among French hybrids, the amount in cultivation was less since the few most widely used hybrids, which were planted on approximately half the cultivated area, were similar for their isozymic and zein chromatographic profiles. Approximately only 50% of the U.S. hybrids were shown to be unique with the remainder classified into groups; at least some of the hybrids within each group could be genetically identical products. Leading U.S. hybrids were unique germplasm but numerous hybrids that collectively covered a large acreage appeared to encompass a relatively narrow genetic base. For the continued success of agriculture, it will be important to improve breeding, registration, and marketing practices in order to ensure the continued supply of genetically different and improved elite hybrids.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-5060
    Keywords: cultivar identification ; electrophoresis ; genetic diversity ; minimum distance ; DNA ; RFLPs ; Zea mays L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Eighty DNA Restriction Fragment Length Polymorphism (RFLP) clones were used as probes to profile 47 hybrids of maize (Zea mays L.) that are of widespread usage in France and 49 hybrids that are either in common usage or are new releases in the U.S. The objectives were to 1) investigate the degree to which RFLPs provide unique characterization of hybrids; 2) show associations among hybrids using both cluster and principal coordinate analyses; 3) measure the ability of RFLPs to show associations among hybrids that reflect those to be expected on the basis of pedigree; and 4) compare the patterns and extent of genetic diversity among French hybrids with that found among a set of widely used U.S. hybrids. RFLPs showed all French hybrids to have different profiles, however, 3 hybrids were very similar with more than 90% of their profiles in common. Twenty-seven U.S. hybrids showed this level of similarity with one or more U.S. hybrids. High correlations (r=0.93, 0.94) were found for pedigree distance versus RFLP distance between pairs of French and of French and U.S. hybrids, respectively. Similar levels of correspondence for rank correlations between RFLP and pedigree data were also found. Similar groupings of hybrids were shown by two cluster analysis methods and by principal coordinate analysis. Inclusion of hybrids in cluster groupings was supported by observation of raw distance data for selected hybrids and their nearest neighbors. Most hectarage in France is planted to hybrids that fall within 2 related groups of germplasm on the basis of RFLP data. Minimum distance standards could promote breeders to surmount the challenge of introducing elite yet diverse germplasm into agriculture.
    Type of Medium: Electronic Resource
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