ISSN:
1617-4623
Keywords:
Key words High-resolution mapping
;
Karyotypeevolution
;
Restriction fragment lengthpolymorphism (RFLP)
;
Zycopersicon
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Several bacteriophage lambda clones containing interstitial telomere repeats (ITR) were isolated from a library of tomato genomic DNA by plaque hybridization with the cloned Arabidopsis thaliana telomere repeat. Restriction fragments lacking highly repetitive DNA were identified and used as probes to map 14 of the 20 lambda clones. All of these markers mapped near the centromere on eight of the twelve tomato chromosomes. The exact centromere location of chromosomes 7 and 9 has recently been determined, and all ITR clones that localize to these two chromosomes map to the marker clusters known to contain the centromere. High-resolution mapping of one of these markers showed cosegregation of the telomere repeat with the marker cluster closest to the centromere in over 9000 meiotic products. We propose that the map location of interstitial telomere clones may reflect specific sequence interchanges between telomeric and centromeric regions and may provide an expedient means of localizing centromere positions.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02173641
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