ISSN:
0886-1544
Keywords:
actin-membrane attachments
;
cytoskeleton
;
α-actinin sequence
;
muscle
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Medicine
Notes:
The dense-bodies in the body wall muscle of the nematode Caenorhabditis elegans function to anchor the actin thin filaments to the adjacent sarcolemma. One of the major components of the dense-bodies is the actin-binding protein α-actinin. To facilitate a genetic analysis of α-actinin, we have cloned a cDNA encoding the nematode protein, identified its position on the nematode physical map, and developed a unique PCR based approach to test the position of the cloned gene relative to known genetic deletions. The peptide sequence deduced from the cDNA shows that, apart from a few exceptional regions, the nematode protein shows strong similarity to other known α-actinins. Its position on the genetic map shows that none of the known muscle affecting mutations identified in C. elegans are in this α-actinin gene. This gene has been given the name atn-1 (α-actinin-1).
Additional Material:
7 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/cm.970200108
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