ISSN:
1573-5044
Keywords:
alfalfa
;
somatic embryogenesis
;
protoplast
;
cell suspensions
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A system was established for achieving plant regeneration from mesophyll protoplasts and cotyledon-derived cell suspension cultures of alfalfa, Medicago sativa L. Peeled leaflets or cells from 6-day-old cell suspensions were incubated in an enzyme mixture containing 1% Driselase, 1% Rhozyme, 0.1% Cellulase and 72 gl-1 mannitol at pH 5.8 for 2–16 h to liberate protoplasts. A complex Kao medium supported cell division and colony formation, whereas a high auxin/low cytokinin treatment on Schenk and Hildebrandt medium followed by culture on growth regulator-free Blaydes or Linsmaier and Skoog medium resulted in somatic embryo formation. Of the three varieties tested. Citation, Answer and Regen S, the latter two produced embryos from which plants could be regenerated.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00033736
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