ISSN:
1432-1041
Keywords:
glipizide
;
radioimmunoassay
;
antibody preparation
;
cross-reactivity
;
precision and accuracy
;
plasma level
;
man
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
,
Medicine
Notes:
Summary A simple, sensitive radioimmunoassay has been developed for the direct determination of glipizide in human plasma. Antisera raised in rabbits immunized with a glipizide analogue conjugated to bovine serum albumin were highly specific, the two main metabolites, 3,cis-hydroxycyclohexyl derivative and 4,trans-hydroxycyclohexyl derivative, having cross reactivities of 0.73% and 1.66%, respectively. The method can measure amounts as small as 1 ng/ml. The intra- and inter-assay coefficients of variation lay between 2.98–5.79% and 2.35–8.66%, respectively. The mean recovery of glipizide added to plasma was 99–105% over the range 1–500 ng/ml. The method was employed to determine plasma levels in six subjects after administration of a 5 mg tablet of glipizide. The results were in accordance with those found after administration of the same dose of radiolabelled glipizide to two other subjects.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00627928
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