ISSN:
1432-0428
Keywords:
Diabetes mellitus
;
pancreatic B-cells
;
serum cytotoxicity
;
serum complement
;
islet cell antibodies
;
rubidium accumulation
;
insulin release
;
autoimmunity
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Serum from normal blood-donors and juvenile diabetic patients inhibited Rb+ accumulation and stimulated release of 51Cr and insulin in suspensions of dispersed pancreatic islet cells prepared from ob/ob mouse islets, which are rich in B-cells. The effects indicate the presence of a B-cytotoxic factor in human serum. Serum from mouse and fetal calf also inhibited the islet cell accumulation of Rb+. Toxicity was not suppressed by treating serum with protein A-Sepharose and did not correlate with islet cell binding of fluorescent antibodies to human immunoglobulin. Whereas all sera inhibited Rb+ accumulation, 3 of 6 diabetic patient sera, but no blood-donor serum, made the cells fluoresce on exposure to the fluorescent antibodies. Supporting a dependence on complement, toxicity remained after dialysis, but was destroyed by treating serum with zymosan-A or heating at 56 ° for 30 min.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00429704
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