ISSN:
1435-1536
Keywords:
Dielectric relaxation
;
scanning dielectric microscope
;
interfacial polarization
;
biological cell
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
,
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
Abstract A dielectric technique that can image local permittivity and conductivity has been applied to living biological cells in an aqueous environment. The local permittivity and conductivity were measured between 10 kHz and 10 MHz with a fine probe electrode, which was laterally scanned over cells on a plate electrode. The dielectric images of the cells depended on frequency, indicating dielectric relaxation that is due to interfacial polarization. The low-frequency image (at ∼10 KHz) in which the cells have high permittivity and low conductivity compared with the medium results from the presence of the plasma membrane with high resistivity. The dielectric image of the cell interior is obtainable at high frequencies (∼10 MHz), where the plasma membrane is short-circuited.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00657679
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