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  • 1990-1994  (2)
  • cadaverine  (1)
  • gas and water cresting  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Expression of a bacterial lysine decarboxylase gene and transport of the protein into chloroplasts of transgenic tobacco (1991)
    Springer
    Plant molecular biology 17 (1991), S. 475-486 
    Details
    ISSN: 1573-5028
    Keywords: Nicotiana tabacum ; plant transformation ; gene expression ; bacterial lysine decarboxylase ; protein transport ; chloroplasts ; cadaverine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A possible approach for altering alkaloid biosynthesis in plants is the expression of genes encoding key enzymes of a pathway such as lysine decarboxylase (ldc) in transgenic plants. Two strategies were followed here: one focused on expression of the gene in the cytoplasm, the other on subsequent targeting of the protein to the chloroplasts. Theldcgene fromHafnia alvei was therefore (a) placed under the control of the 1′ promoter of the bidirectional Tr promoter fromAgrobacterium tumefaciens Ti- plasmid, and (b) cloned behind therbcS promoter from potato fused to the coding region of therbcS transit peptide. Bothldc constructs, introduced intoNicotiana tabacum with the aid ofA. tumefaciens, were integrated into the plant genome and transcribed as shown by Southern and northern hybridization. However, LDC activity was only detectable in plants expressing mRNA under the control of therbcS promoter directing the LDC fusion protein into chloroplasts with the aid of the transit peptide domain. In plants expressing the processed bacterial enzyme cadaverine levels increased from nearly zero to 0.3–1% of dry mass.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00040641
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Analytical solutions of 2D cresting models using the hodograph method (1994)
    Springer
    Transport in porous media 15 (1994), S. 251-269 
    Details
    ISSN: 1573-1634
    Keywords: Hodograph method ; 2D cresting models ; gas and water cresting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Technology
    Notes: Abstract Three new applications of the hodograph method to the problem of gas and water cresting towards horizontal wells are presented: (1) A numerical technique, based on the hodograph method, is used to find the shape of the oil/water interface and values of critical heights for the problem of water cresting towards a horizontal well beneath an impermeable plane. (2) By correct implementation of the boundary conditions in the lateral edge drive model, a hodograph solution is found for which the water crest tends to a horizontal asymptote far from the well, rather than tending towards a parabolic curve as in previous approaches. The solution yields integral representations for the lengths of boundary segments and enables an explicit expression for the critical rate to be derived in terms of the distance to the constant potential boundary. (3) The problem of simultaneous gas and water cresting towards a horizontal well in a thin oil column reservoir is solved using the hodograph method, providing shapes of the free interfaces and an expression for the optimal placement of the well with respect to the interfaces.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00613281
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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