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  • 1
    Digitale Medien
    Digitale Medien
    Enhancement of nuclear Ca2+-ATPase activity in regenerating rat liver: involvement of nuclear DNA increase (1995)
    Springer
    Molecular and cellular biochemistry 146 (1995), S. 179-186 
    Details
    ISSN: 1573-4919
    Schlagwort(e): Ca2+-ATPase ; calcium ; nuclear DNA ; DNA fragmentation ; regucalcin ; regenerating rat liver
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract The alteration of calcium content, Ca2+-ATPase activity, DNA content and DNA fragmentation in the nuclei of regenerating rat liver was investigated. Liver was surgically removed about 70% of that of sham-operated rats. the reduced liver weight by partial hepatectomy was completely restored at 3 days after the surgery. Regenerating liver significantly increased Ca2+-ATPase activity and DNA content in the nuclei between 1 and 5 days after hepatectomy. The nuclear calcium content was clearly increased from 2 days after hepatectomy. The increase of Ca2+-ATPase activity in regenerating liver was clearly inhibited by the presence of trifluoperazine (10 μM), staurosporine (2.5 μM) and dibucaine (10 μM), which are inhibitors of calmodulin and protein kinase, in the enzyme reaction mixture. However, the nuclear enzyme activity in normal rat liver was not significantly altered by these inhibitors. Meanwhile, the increase of nuclear DNA content in regenerating liver was completely blocked by the administration of trifluoperazine (2.5 mg/100 g body weight), suggesting an involvement of calmodulin. Now, the nuclear DNA fragmentation was significantly decreased in regenerating liver, suggesting that this decrease is partly contributed to the increase in nuclear DNA content. The present study clearly demonstrates that regenerating liver enhances nuclear Ca2+-ATPase activity and induces a corresponding elevation of nuclear calcium content. This Ca2+-signaling system may be involved in the regulation of nuclear DNA functions in regenerating rat liver.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00944611
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  • 2
    Digitale Medien
    Digitale Medien
    Characterization of regucalcin effect on proteolytic activity in rat liver cytosol: relation to cysteinyl-proteases (1995)
    Springer
    Molecular and cellular biochemistry 148 (1995), S. 67-72 
    Details
    ISSN: 1573-4919
    Schlagwort(e): regucalcin ; calcium ; protease ; calpain ; rat liver cytosol
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract The increasing effect of regucalcin, isolated from rat liver cytosol, on neutral proteolytic activity in the hepatic cytosol was characterized. The proteolytic activity was markedly elevated by the addition of regucalcin (0.1–0.5 μM) in the absence of Ca2+. This increase was not significantly altered by the presence of diisopropylfluorophsophate (DPF;2.5 mM)—although DFP caused a significant decrease in the proteolytic activity. Regucalcin (0.25 μM) additively enhanced the dithiothreitol (DTT; 1.0 mM)—increased proteolytic activity, while the regucalcin or DTT effect was completely abolished by NEM (5 mM), indicating that regucalcin may act on the SH group in proteases. Also, regucalcin (0.25 μM) enhanced the effect of Ca2+ (10 μM) increasing liver proteolytic activity, suggesting that regucalcin does not influence on the active sites for Ca2+ in proteases. Moreover, the proteolytic activity of regucalcin (0.25 μM) was significantly decreased by the presence of calpastatin (24 μg/ml), an inhibitor of Ca2+-activated neutral protease (calpain). Now, regucalcin (0.25 μM) increased about 7-fold the activity ofm-calpain isolated from rabbit skeletal muscle. These observations demonstrate that regucalcin directly activates cysteinyl-proteases. Regucalcin may have a role as a potent proteolytic activator in the cytoplasm of liver cells.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00929504
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  • 3
    Digitale Medien
    Digitale Medien
    Inhibitory effect of calcium-binding protein regucalcin on ribonucleic acid synthesis in isolated rat liver nuclei (1997)
    Springer
    Molecular and cellular biochemistry 173 (1997), S. 169-175 
    Details
    ISSN: 1573-4919
    Schlagwort(e): regucalcin ; calcium-binding protein ; calcium ; nuclear RNA synthesis ; regenerating ratliver
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract The effect of regucalcin, a Ca2+-bindingf protein isolated from rat livercytosol, on ribonucleic acid (RNA) synthesis in the nuclei of normal ratliver and of regenerating rat liver was investigated. The liver weight at 1day after partial hepatectomy was increased about 50% of that ofsham-operated (control) rats. Calcium chloride (1.0-20 µM Ca2+ asfinal concentration) was added into the reaction mixture of nuclear RNAsynthesis. RNA synthesis was established by incorporation of [3H]-uridine5'-triphosphate (UTP) into the nuclear RNA. Addition of Ca2+ (5 and 10µM) caused a significant increase of RNA synthesis in the nuclei fromcontrol rat liver. Such effect of Ca2+ was potentiated in the nuclei ofregenerating liver; nuclear RNA synthesis was increased about 2 fold by the1.0 and 2.5 µM Ca2+ addition. The stimulatory effect of Ca2+ wassignificantly inhibited by the presence of a-amanitin (10-8 M), an inhibitorof RNA polymerase II. The presence of regucalcin (0.25 and 0.5 µM)significantly inhibited RNA synthesis in the nuclei from control rat liverand from regenerating rat liver. The inhibitory effect of regucalcin wasremarkable in the presence of EGTA (0.5 mM), and it was weakened by theaddition of Ca2+ (5 µM). Such regucalcin effect was not seen in thepresence of a-amanitin. The presence of anti-regucalcin IgG in the reactionmixture significantly increased RNA synthesis in the nuclei from control ratliver, indicating that the endogenous regucalcin may be involved in nuclearRNA synthesis. The present resuits demonstrate that regucalcin can inhibitnuclear RNA synthesis in rat liver. Regucalcin may have an inhibitory rolein the regulation of liver nuclear RNA synthesis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1006833111236
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  • 4
    Digitale Medien
    Digitale Medien
    Streptozotocin-induced diabetes increases (Ca2+-Mg2+)-ATPase activity in hepatic plasma membranes of rats: Involvement of protein kinase C (1998)
    Springer
    Molecular and cellular biochemistry 178 (1998), S. 311-316 
    Details
    ISSN: 1573-4919
    Schlagwort(e): diabetes ; Ca2+-Mg2+-ATPase ; calcium ; liver plasma membrane
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract The alteration in calcium transport in the liver of rats with streptozocin(STZ)-diabetic state was investigated. STZ (6 mg/100 g body weight) was subcutaneously administered in rats, and 1 or 2 weeks later they were sacrificed by bleeding. STZ administration caused a remarkable elevation of serum glucose concentration. Liver calcium content was significantly increased by STZ administration. Hepatic plasma membrane (Ca2+-Mg2+)-ATPase activity was markedly elevated by STZ administration. This increase was completely abolished by the presence of staurosporine (10-7-10-5 M), an inhibitor of protein kinase C, in the enzyme reaction mixture, suggesting an involvement of protein kinase C signalling. Moreover, the STZ-induced increase in liver plasma membrane (Ca2+-Mg2+)-ATPase activity was significantly raised by the presence of okadaic acid (10-5 and 10-4 M). Meanwhile, the STZ-increased (Ca2+-Mg2+)-ATPase activity was not appreciably altered by the presence of anti-regucalcin IgG in the reaction mixture, indicating that the activatory protein regucalcin does not participate in the elevation of the enzyme activity. The present study demonstrates that STZ-induced diabetes causes the increase in hepatic plasma membrane (Ca2+-Mg2+)-ATPase activity of rats.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1006871615498
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  • 5
    Digitale Medien
    Digitale Medien
    Effect of anti-regucalcin antibody on neutral phosphatase activity in rat liver cytosol: Involvement of endogenous regucalcin (1999)
    Springer
    Molecular and cellular biochemistry 197 (1999), S. 25-29 
    Details
    ISSN: 1573-4919
    Schlagwort(e): regucalcin ; anti-regucalcin antibody ; protein phosphatase ; calcium ; rat liver cytosol
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract The effect of anti-regucalcin monoclonal antibody on neutral phoshatase activity in rat liver cytosol was investigated. Phosphotyrosine, phosphoserine, and phosphothreonine were used as the substrate toward phosphatase asssy. Liver cytosolic phosphatase activity with three phosphoaminoacids was significantly increased in the presence of anti-regucalcin antibody (100 and 200 ng/ml) in the enzyme reaction mixture with calcium chloride (0.1 mM) or EGTA (1.0 mM). The effect of anti-regucalcin antibody was completely abolished in the presence of exogenous regucalcin (1.0 μM), indicating the involvement of endogenous regucalcin. The anti-regucalcin anti body- increased phosphatase activity was not significantly altered in the presence of trifluoperazine (20 μM), an antagonist of calmodulin, or akadaic acid (10 μM), an inhibitor of protein phosphatase, although these inihibitors caused a slight decrease in liver cytosolic phosphatase activity. The effect of endogenous regucalcin might be not related to calmodulin, and it was insensitive to okadaic acid. The present findings suggest that endogenous regucalcin is involved in the regulation of protein phasphatase in rat liver cytoplasm.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1006979606225
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  • 6
    Digitale Medien
    Digitale Medien
    Reversible effect of calcium-binding protein regucalcin on the Ca2+-induced inhibition of deoxyuridine 5′-triphosphatase activity in rat liver cytosol (1992)
    Springer
    Molecular and cellular biochemistry 110 (1992), S. 25-29 
    Details
    ISSN: 1573-4919
    Schlagwort(e): calcium ; regucalcin ; deoxyuridine 5′-triphosphatase ; rat liver cytosol
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract The effect of regucalcin, a calcium-binding protein isolated from rat liver cytosol, on deoxyuridine 5′-triphosphatase (dUTPase) in the cytosol of rat liver was investigated. Addition of Ca2+ up to 5.0 µM to the enzyme reaction mixture caused a significant decrease of dUTPase activity, while Zn2+, Cd2+, Co2+, Al3+, Mn2+ and Ni2+ (10 µM) did not have an appreciable effect. The Ca2+-induced decrease of dUTPase activity was reversed by the presence of regucalcin; the effect was complete at 1.0 µM of the protein. Regucalcin had no effect on the basal activity of the enzyme. Meanwhile, the reversible effect of regucalcin on the Ca2+ (10 µM)-induced decrease of dUTPase activity was not altered by the coexistence of Cd2+ or Zn2+ (10 µM). The present data suggest that liver cytosolic dUTPase is uniquely regulated by Ca2+ of various metals, and that the Ca2+ effect is reversed by regucalcin.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02385002
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  • 7
    Digitale Medien
    Digitale Medien
    Expression of hepatic calcium-binding protein regucalcin mRNA is elevated by refeeding of fasted rats: Involvement of glucose, insulin and calcium as stimulating factors (1995)
    Springer
    Molecular and cellular biochemistry 142 (1995), S. 35-41 
    Details
    ISSN: 1573-4919
    Schlagwort(e): regucalcin ; calcium-binding protein ; insulin ; calcium ; gene expression ; rat liver
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract The effect of refeeding on the expression of Ca2+-binding protein regucalcin mRNA in the liver of fasted rats was investigated. When rats were fasted overnight, the hepatic regucalcin mRNA level was reduced about 70% of that in feeding rats. Refeeding produced a remarkable elevation of hepatic regucalcin mRNA level (about 150–170% of fasted rats). Liver regucalcin concentration was appreciably increased by refeeding, although it was not altered by fasting. The oral administration of glucose (2 g/kg body weight) to fasted rats caused a significant increase in hepatic regucalcin mRNA level. Moreover, hepatic regucalcin mRNA level was clearly elevated by a single subcutaneous administration of insulin (10 and 100 U/kg) to fasted rats. The hormonal effect was not further enhanced by the simultaneous administration of calcium chloride (250 mg Ca/kg) to fasted rats, although calcium administration stimulated regucalcin mRNA expression in the liver. The present study suggests that the expression of hepatic regucalcin mRNA stimulated by refeeding is significantly involved in the action of insulin and/or calcium as stimulating factors.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00928911
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  • 8
    Digitale Medien
    Digitale Medien
    Increase of (Ca2+−Mg2+)-ATPase activity in hepatic plasma membranes of rats administered orally calcium: The endogenous role of regucalcin (1995)
    Springer
    Molecular and cellular biochemistry 144 (1995), S. 1-6 
    Details
    ISSN: 1573-4919
    Schlagwort(e): regucalcin ; (Ca2+−Mg2+)-ATPase ; calcium pump ; plasma membrane ; rat liver
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract The alteration of the plasma membrane (Ca2+−Mg2+)-ATPase activity in the liver of rats administered orally calcium chloride solution was investigated. The plasma membrane (Ca2+−Mg2+)-ATPase activity was significantly increased by a single oral administration of calcium (10, 25 and 50 mg/100 g body weight) in rats. This increase was seen between 10 and 60 min after the administration. The presence of anti-regucalcin IgG (1.0–5.0 μg/ml) in the enzyme reaction mixture caused a complete inhibition for the elevation of the plasma membrane (Ca2+−Mg2+)-ATPase activity by the addition of regucalcin (0.25 μM). Also, the calcium administration-induced increase in hepatic plasma membrane (Ca2+−Mg2+)-ATPase activity was completely abolished by the presence of anti-regucalcin IgG (1.0 and 2.5 μg/ml). Moreover, the calcium administration-induced increase in hepatic plasma membrane (Ca2+−Mg2+)-ATPase activity was not inhibited by vanadate (0.1 and 0.2 mM) addition into the enzyme reaction mixture, although the inhibitory effect of vanadate was seen in the plasma membranes from normal rat liver. Now, the activating effect of regucalcin (0.25 μM) on hepatic plasma membrane (Ca2+−Mg2+)-ATPase was not inhibited by vanadate addition. The endogenous regucalcin may play a role in the calcium administration-induced increase of (Ca2+−Mg2+)-ATPase activity in the liver plasma membranes of rats.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00926733
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  • 9
    Digitale Medien
    Digitale Medien
    Suppressed expression of calcium-binding protein regucalcin mRNA in the renal cortex of rats with chemically induced kidney damage (1995)
    Springer
    Molecular and cellular biochemistry 151 (1995), S. 55-60 
    Details
    ISSN: 1573-4919
    Schlagwort(e): regucalcin ; calcium ; gene expression ; kidney damage ; rat kidney cortex
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract The alteration of Ca2+-binding protein regucalcin mRNA expression in the kidney cortex of rats administered cisplatin and cephaloridine, which can induce kidney damage, was investigated. Cisplatin (0.25, 0.5 and 1.0 mg/100 g body weight) or cephaloridine (25, 50 and 100 mg/100 g) was intraperitoneally administered in rats, and 1, 2 and 3 days later they were sacrificed. The alteration in serum findings after the administration of cisplatin (1.0 mg/100 g) or cephaloridine (50 and 100 mg/100 g) demonstrated chemically induced kidney damage; blood urea nitrogen (BUN) concentration increased markedly and serum inorganic phosphorus or calcium concentration decreased significantly. Moreover, the administration of cisplatin (1.0 mg/100 g) or cephaloridine (100 mg/100 g) caused a remarkable increase of calcium content in the kidney cortex of rats, indicating kidney damage. The expression of regucalcin mRNA in the kidney cortex was markedly reduced by the administration of cisplatin or cephaloridine in rats, when the mRNA levels were analyzed by Northern blotting using rat liver regucalcin cDNA (0.9 kb). The mRNA decreases were seen with the used lowest dose of cisplatin or cephaloridine. The present study clearly demonstrates that the mRNA expression of Ca2+-binding protein regucalcin in the kidney cortex of rats is decreased by chemically induced kidney damage.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01076896
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  • 10
    Digitale Medien
    Digitale Medien
    Inhibitory effect of calcium-binding protein regucalcin on Ca2+/calmodulin-dependent cyclic nucleotide phosphodiesterase activity in rat liver cytosol (1991)
    Springer
    Molecular and cellular biochemistry 106 (1991), S. 25-30 
    Details
    ISSN: 1573-4919
    Schlagwort(e): calcium ; regucalcin ; calmodulin ; cyclic nucleotide phosphodiesterase ; rat liver
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract The effect of regucalcin, a calcium-binding protein isolated from rat liver cytosol, on Ca2+/calmodulin-dependent cyclic nucleotide (AMP) phosphodiesterase activity in rat liver cytosol was investigated. The addition of Ca2+ (50 µM) and calmodulin 160 U/ml in the enzyme reaction mixture caused a significant increase in cyclic AMP phosphodiesterase activity. This increase was inhibited by the presence of regucalcin (0.5-3.0 µM); the inhibitory effect was complete at 1.0 µM. Regucalcin (1.0 µM) did not have an appreciable effect on basal activity without Ca2+ and calmodulin. The inhibitory effect of regucalcin was still evident even at several fold higher concentrations of calmodulin (160–480 U/ml). However, regucalcin (1.0 µM) did not inhibit Ca2+/calmodulin-dependent cyclic AMP phosphodiesterase activity in the presence of 100 and 200 µM Ca2+ added. Meanwhile, Cd2 (25–100 µM)-induced decrease in Ca2+/calmodulin-dependent cyclic AMP phosphodiesterase activity was not reversed by the presence of regucalcin (1.0 µM). The present results suggest that regucalcin can regulate Ca2+/calmodulin-dependent cyclic AMP phosphodiesterase activity due to binding Ca2+ in liver cells.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00231185
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