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  • 1
    Electronic Resource
    Electronic Resource
    In vitro morphogenetic capacity of Nicotiana tabacum L. var. BEL W3 upon cyclic culture and regeneration (1994)
    Springer
    Euphytica 76 (1994), S. 7-12 
    Details
    ISSN: 1573-5060
    Keywords: Nicotiana tabacum var. BEL W3 ; tobacco ; callus ; morphogenetic capacity ; somaclone ; epigenetic
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Variation in the capacity to regenerate shoots and morphogenetic potential were investigated by cyclic culture and regeneration of Nicotiana tabacum var. BEL W3. Explants were excised from different shoots formed by the same callus line. Differences in the organogenetic in vitro response among regenerated plantlets was observed in two subsequent regenerate ‘generations’. A progressive decrease of the regenerative capacity was observed across callus line ‘generations’.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00024015
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Manganese toxicity to different growth processes in vitro in Nicotiana (1997)
    Springer
    Plant cell, tissue and organ culture 50 (1997), S. 125-129 
    Details
    ISSN: 1573-5044
    Keywords: callus ; metal tolerance ; shoot regeneration ; Solanaceae ; tobacco
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Manganese toxicity to germination, callus induction and shoot regeneration was studied on three cultivars of Nicotiana tabacum: BEL W3, Burley 21, Bright NC 944. All materials were cultured on MS solid medium containing 0.1 (control), 2, 5, 10, 15 and 20 mM Mn, to which 5 µM NAA and 5 µM kinetin were added for callus induction and shoot regeneration. Mn toxicity to callus growth was tested using habituated callus of Nicotiana bigelovii var. bigelovii grown on MS medium without growth regulators. Mn concentrations higher than 2 mM were toxic for germination, and concentrations higher than 5 mM were toxic for callus induction, shoot regeneration and callus growth. Among the cultivars examined, Bright tobacco appeared more tolerant to high Mn concentrations during callus formation and shoot regeneration. However, many regenerated plants capable of growing in vitro in the presence of 2 and 5 mM Mn were obtained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005917114146
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    In vitro culture of Foeniculum vulgare: callus characteristics in relation to morphogenesis (1996)
    Springer
    Plant cell, tissue and organ culture 45 (1996), S. 263-268 
    Details
    ISSN: 1573-5044
    Keywords: Auxin ; callus ; cytokinin ; fennel ; histological analysis ; plant regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Callus induction and morphogenic response of several fennel populations were determined by genotype and hormonal treatment. 100% callus formation occurred only in the Francia Pernod population under the action of 2, 4-dichlorophenoxyacetic acid or α-naphthaleneacetic acid and kinetin. Only this last hormonal treatment induced shoot regeneration. Plant regeneration was observed especially in Francia Pernod population. Ths calluses grown in presence of 2,4-dichlorophenoxyacetic acid or α-naphthaleneacetic acid plus kinetin, showed considerable differences at cytological and histological level which were correlated to their different morphogenic capability.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00043640
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    In vitro culture of species and varieties of four Amaranthus L. species (1992)
    Springer
    Euphytica 62 (1992), S. 181-186 
    Details
    ISSN: 1573-5060
    Keywords: Amaranthus spp. ; callus ; organogenesis ; species ; varieties ; amaranthus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Callus induction, callus growth and organogenetic processes were investigated in hypocotyl and stem cultures of four species of Amaranthus each of which comprised several varieties. Callus formation occurred in almost all the varieties in 100% of explants. The combinations of naphtaleneacetic acid plus benzylaminopurine or 2,4-dichlorophenoxyacetic acid plus Kinetin were very effective in causing callus formation; the results for callus growth in presence of the same growth regulator combinations revealed in some cases significant differences among the varieties. As far organogenesis-based on a few varieties-only A. caudatus and A. hypochondriacus responded well forming shoots from callus when cultured in presence of indoleacetic acid plus Kinetin and/or indoleacetic acid plus benzylaminopurine. Root regeneration was also observed in several varieties. Complete plantlets were obtained from rooted shoots. The results are discussed in relation to hormonal effect and the genotype importance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00041752
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Response of shape in vitro cultures of shape Nicotiana tabacum L. to copper stress and selection of plants from Cu-tolerant callus (1998)
    Springer
    Plant cell, tissue and organ culture 53 (1998), S. 161-169 
    Details
    ISSN: 1573-5044
    Keywords: callus ; copper toxicity ; N. tabacum ; plant regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In Nicotiana tabacum L. var. BEL W3 copper (Cu) at concentrations higher than 50 μM significantly inhibited callus growth and shoot regeneration. After 5–6 months of culture only a few morphogenic callus lines survived in the presence of 100 μM Cu. These calluses showed the capacity to grow and regenerate shoots through successive subcultures on medium containing 100 μM Cu. The 100 μM Cu-tolerant shoots, in comparison to regenerated control shoots, formed roots only when cultured in the presence of 100 μM Cu. From five independent Cu-tolerant callus lines in a culture period of 4–5 months more than 50 plants (defined ‘tolerant’) able to grow in presence of 100 μM Cu were obtained. These plants showed normal xylem tissue formation while control regenerated plants growing in normal Cu MS content (0.1 μM) had few xylem elements in the central cylinder. No difference as far chlorophyll content and chloroplast structure was found among Cu-tolerant and control plants. In Cu-tolerant plants, dry matter production was higher than in controls, particularly in roots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006048031956
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    Paper (German National Licenses)
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