ISSN:
0268-2575
Keywords:
agitation and aeration rates
;
growth phase
;
cellulase-free xylanases
;
cellular disintegration
;
enzyme release phase
;
Thermomyces lanuginosus
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Thermomyces lanuginosus RT9 was grown in a stirred and aerated reactor for the production of xylan-degrading enzymes and the kinetics of xylanase and β-xylosidase secretion investigated. During each fermentation, kLa decreased with increasing mycelia concentration to the point of cellular disintegration. Although both agitation and aeration rates had significant influences on the growth rate as well as on the yields and rates of enzymes (xylanase and β-xylosidase) release, the enzyme secretion kinetics showed a comparable pattern independent of the operating conditions. Enzyme secretion could be separated into a growth phase, during which extracellular xylanase was at low level, and an enzyme release phase, when there was a notable increase in the xylanase production rate accompanying cellular disintegration.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jctb.280630306
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