ISSN:
1570-7458
Keywords:
Brassica fruticulosa
;
Brassica spinescens
;
Brevicoryne brassicae
;
lectin
;
host-plant resistance
;
chitin
;
artificial diets
;
chitinase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A lectin, with a chitin-binding domain and chitinase activity, is present in significant quantities in the wild brassica speciesB. fruticulosa andB. spinescens but at low levels in cultivated cabbage cv. Offenham Compacta. The lectin, purified 〉1000 fold after binding to chitin, migrated on SDS-PAGE gels as a single band with a M r of 14.500. The amino acid composition of the lectin fromB. spinescens indicated high concentrations of asparagine/aspartic acid, glycine, leucine and serine in common with other chitin-binding lectins with insecticidal and antifungal activities. Brassica lectin and the closely related agglutinin from wheatgerm and nettle show significant insecticidal activity when presented toBrevicoryne brassicae in chemically-defined synthetic diets.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02383553
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