ISSN:
1573-5079
Keywords:
green sulfur bacteria
;
chlorosome
;
bacteriochlorophyll c
;
bacteriochlorophyll d
;
bacteriochlorophyll e
;
HPLC
;
absorption spectrum
;
mass spectroscopy
;
Chlorobiaceae
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The chlorosomal bacteriochlorophyll (BChl) composition of the green sulfur bacteria Chlorobium vibrioforme and Chlorobium phaeovibrioides was investigated by means of normal-phase high-performance liquid chromatography. From both species a number of homologues was isolated, which were identified by absorption and 252Cf-plasma desorption mass spectroscopy. Besides BChl d, C. vibrioforme contained a significant amount of BChl c, which may provide an explanation for the previous observation of at least two spectrally different pools of BChl in the chlorosomes of green sulfur bacteria (Otte et al. 1991). C. phaeovibrioides contained various homologues of BChl e only. Absorption spectra in acetone of BChl c, d and e, as well as bacteriopheophytin e are presented. No systematic differences were found for the various homologues of each pigment. In addition to farnesol, the mass spectra revealed the presence of various minor esterifying alcohols in both species, including phytol, oleol, cetol and 4-undecyl-2-furanmethanol, as well as an alcohol of low molecular mass, which is tentatively assumed to be decenol.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00014746
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