ISSN:
0006-3592
Schlagwort(e):
liposomes
;
vesicles
;
microreactor
;
permeability
;
chymotrypsin
;
enzyme
;
Chemistry
;
Biochemistry and Biotechnology
Quelle:
Wiley InterScience Backfile Collection 1832-2000
Thema:
Biologie
,
Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
Notizen:
Liposomes were prepared from 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), which contained the water soluble proteinase α-chymotrypsin. This liposome entrapped enzyme showed selectivity for externally added substrates in that only small substrates (benzoyl-l-Tyr-p-nitroanilide or acetyl-l-Phe-p-nitro-anilide) - for which the liposome bilayer was permeable - were transformed into products. Large substrates (succinyl-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide or casein) could not penetrate from the external aqueous phase into the liposomes, and were not hydrolyzed. This substrate selectivity is entirely based on the compartimentation and permeability properties of the liposome microreactor. © 1998 John Wiley & Sons, Inc.Biotechnol. Bioeng. 57: 216-219, 1998.
Zusätzliches Material:
5 Ill.
Materialart:
Digitale Medien
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