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Digitale Medien

A first estimate of organic carbon storage in Holocene lake sediments in Alberta, Canada (2000)

Campbell, I.D. ; Campbell, C. ; Vitt, D.H. ; [weitere]

Springer

Journal of paleolimnology 24 (2000), S. 395-400

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ISSN: 1573-0417

Schlagwort(e): carbon storage ; lake sediment ; Holocene ; Canada ; climate change ; organic matter

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Geologie und Paläontologie

Notizen: Abstract This paper reports a first estimate of the Holocene lake sediment carbon pool in Alberta, Canada. The organic matter content of lake sediment does not appear to depend strongly on lake size or other limnological parameters, allowing a simple first estimate in which we assume all Alberta lake sediment to have the same organic matter content. Alberta lake sediments sequester about 15 g C m-2 yr-1, for a provincial total of 0.23 Tg C yr-1, or 2.3 Pg C over the Holocene. Alberta lakes may represent as much as 1/1700 of total global, annual permanent carbon sequestration.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1023/A:1008103605817

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Digitale Medien

Particle-mediated gene transfer to rat neurons in primary culture (2000)

Usachev, Y.M. ; Khammanivong, A. ; Campbell, C. ; [weitere]

Springer

Pflügers Archiv 439 (2000), S. 730-738

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ISSN: 1432-2013

Schlagwort(e): Dorsal root ganglion neurons Gene gun Green fluorescent protein Hippocampal neurons Particle-mediated gene transfer Primary tissue culture Transfection

Quelle: Springer Online Journal Archives 1860-2000

Thema: Medizin

Notizen: Abstract. Gene transfer into neuronal cells provides an important approach to study their function. Particle-mediated gene delivery was used to transfect rat dorsal root ganglion (DRG) and hippocampal neurons in primary culture with the genes for the enhanced blue and green fluorescent proteins (EBFP and EGFP) under control of the cytomegalovirus promoter. Quantitative analysis of marker protein fluorescence detected expression at 3 h that continued to increase for 48 h. For DRG neurons the optimal expression efficiency of 8±2% was obtained 24 h following transfection. In contrast, approximately 2±1% of hippocampal neurons in culture expressed EGFP at 3 h which subsequently declined. Co-transfection of DRG cultures with two plasmids produced reliable expression of both genes. Transfected DRG neurons exhibited normal electrophysiological properties, and resting and stimulated intracellular Ca2+ concentrations were unchanged. After transfection, 44% of hippocampal neurons remained in functional synaptic networks as indicated by glutamatergic Ca2+ spiking activity. Particle-mediated gene delivery provided a straightforward, reproducible and efficient method for transfection of neurons in primary culture. Transfected cells were easily identified by EGFP fluorescence, enabling subsequent physiological analysis. Biolistic particle bombardment was well tolerated by peripheral neurons, although caution was required when this method was applied to CNS cultures.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/s004249900240

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