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  • 1
    Electronic Resource
    Electronic Resource
    RAPD markers linked to a gene for resistance to pine needle gall midge in Japanese black pine (Pinus thunbergii) (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 391-395 
    Details
    ISSN: 1432-2242
    Keywords: Key words Pinus thunbergii ; Pine needle gall midge ; RAPD ; Bulked segregant analysis ; Linkage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Linkage of RAPD markers to a single dominant gene for resistance to pine needle gall midge was investigated in Japanese black pine (Pinus thunbergii). Three primers that generated linked markers were found after 1160 primers were screened by bulked segregant analysis. The distances between the resistance gene, R, and the marker genes OPC06580, OPD01700, and OPAX192100 were 5.1 cM, 6.7 cM and 13.6 cM, respectively. OPC06580 was in coupling phase to R, whereas OPD01700 and OPAX192100 were in repulsion phase to R. A linkage map for a resistant tree was constructed using 96 macrogametophytes. In linkage analysis, 98 out of 127 polymorphic markers were assigned to 17 linkage groups and six linked pairs. The total length of this map was 1469.8 cM, with an average marker density of 15.6 cM. The genome length was estimated to be 2138.3 cM, and the derived linkage map covered 67.5% of the genome. Although the linked markers OPC06580, OPAX192100, and OPD01700, belonged to the same linkage group, no precise positions were found for OPC06580 or OPD01700.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050051
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  • 2
    Electronic Resource
    Electronic Resource
    A puhA gene deletion and plasmid complementation system for facile site directed mutagenesis studies of the reaction center H protein of Rhodobacter sphaeroides (1998)
    Springer
    Photosynthesis research 55 (1998), S. 369-373 
    Details
    ISSN: 1573-5079
    Keywords: complementation ; deletion ; puhA gene ; reaction center H protein ; Rhodobacter sphaeroides ; site directed mutagenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We describe the development of a Rhodobacter sphaeroides RC H (puhA) gene deletion/plasmid complementation system for expression of site directed mutants of the RC H protein. The mutant strain ΔPUHA was constructed by introduction of a translationally in-frame deleted puhA allele at the chromosomal puhA gene site, and evaluated in plasmid complementation experiments. Strain ΔPUHA was not capable of photosynthetic growth, and SDS-PAGE chromatophore proteins confirmed the absence of the RC H protein band. When ΔPUHA was complemented with the wild type puhA gene in plasmids, photosynthetic growth and the RC H protein band in SDS-PAGE were restored. The results of comparisons of the properties of strains with different types of chromosomal puhA gene disruptions in complementation experiments with plasmid-borne DNA fragments containing the wild type puhA gene are consistent with the idea that expression of one or more genes located 3′ of puhA is required for optimal RC levels and photosynthetic growth. Since the ΔPUHA translationally in frame deletion does not seem to interfere with transcription through and beyond the residual puhA sequences, this strain allows facile evaluation of the consequences of plasmid-borne RC H mutations in an otherwise wild type genetic background. Two plasmid-borne site directed mutants of the puhA gene (GluH173 → Gln and GluH173 → Asp) exhibited different deficiencies in photosynthetic growth when present in ΔPUHA, indicating that a carboxylic acid amino acid side chain at the H173 position is important for RC function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005983903558
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    Paper (German National Licenses)
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