ISSN:
0730-2312
Keywords:
platelets
;
morphological change
;
[Ca2+]i
;
confocal laser scanning microscopy
;
surface contact activation
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
Notes:
The dynamic change of cytoplasmic Ca2+ concentration ([Ca2+]i) and morphological change were investigated simultaneously by confocal laser scanning microscopy using fluo-3 and by differential interference contrast optics in platelets activated by contact with the following types of surfaces: native glass and glass treated with poly-L-lysine (PLL), fibrinogen (Fg), or von Willebrand factor (vWF). The initial [Ca2+]i values just after the surface contact were comparable (approximately 100 nM) among platelets deposited on the four surface types. On the PLL-surface, no morphological change or [Ca2+]i elevation was observed. Glass-, Fg-, and vWF-surface adhered platelets showed pseudopod formation and spreading associated with the inhomogeneous [Ca2+]i rise. The platelets on the Fg-surface were the most active in terms of [Ca2+]i rise and morphological change. During pseudopod formation, the mean [Ca2+]i value was maximal and localized high [Ca2+]i zones were observed inside pseudopods, as well as in the center of the platelets. After spreading, high [Ca2+]i zones still remained in the center of the cell. This new technique enabled simultaneous observation of [Ca2+]i and cell shape and we clearly demonstrated a close relationship between [Ca2+]i and morphological alterations. © 1996 Wiley-Liss, Inc.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
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