ISSN:
1573-7330
Keywords:
culture medium
;
embryo
;
fertilization
;
interferon γ
;
interleukin 10
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Purpose The aim of our study is to elucidate whether human oocyteslembryos secrete IFN γ and/or IL-10 and whether the fertilization process depends on the balance between these cytokines. Methods A total of 142 embryo culture media from 24 patients were collected and the cytokine levels were tested with ELISA. Results IFN γ and IL-10 were detectable in 40.1% and 29.6% of culture media respectively. The difference of IFN γ and IL-10 levels in media from fertilized oocytes between day 1 and day 2 are significant (0.46 vs. 1.47 and 34.2 vs. 12.7, respectively). However there was no significant difference between the IFN γ levels of the media from fertilized and nonfertilized oocytes 0.46 vs. 0.85 at day 1 and 1.47 vs. 1.49 at day 2, as well as IL-10 levels 34.2 vs. 30.9 at day 1 and 12.7 vs. 9.58 at day 2 respectively. Conclusions Human preimplantation embryos secrete the cytokines IFN γ and IL-10. No effect of these cytokines on fertilization process could be shown.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02212580
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