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Human pancreatic islet tumor cells maintained in vitro (1973)

Yip, C. C. ; Schimmer, B. P.

Springer

Diabetologia 9 (1973), S. 251-254

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ISSN: 1432-0428

Keywords: Human insulinoma ; culture of dissociated tumorβ-cells ; insulin release in culture ; stimulation of insulin release by cyclic-AMP and tolbutamide

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Summary Tumor cells from two human insulinomas were maintained in culture for more than 6 months. The cultured cells synthesized and secreted proinsulin and insulin; and responded to cyclic AMP (1 mM) and tolbutamide (1 mM) stimulation with increased insulin release. In culture, these cells did not respond to stimulation by high concentrations of glucose (300 mg%) or by glucagon (10 μg/ml).

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01221850

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