ISSN:
0884-3996
Schlagwort(e):
·O-2 extraction
;
dimethyl sulphoxide
;
differential pulse polarography
;
electron spin resonance
;
luminescence
;
Chemistry
;
Biochemistry and Biotechnology
Quelle:
Wiley InterScience Backfile Collection 1832-2000
Thema:
Biologie
,
Chemie und Pharmazie
Notizen:
Extraction of whole lobes of normal rat liver with dimethyl sulphoxide (DMSO) under N2 gives extracts which contain 5 - 10 μmol/l·O-2 (50-100 nmol·O-2 per 10 ml extract per 4 g liver; 1.25-2.50 nmol·O-2 per millilitre per gram liver). Evidence for ·O-2 in the extracts is given by: (1) electron spin resonance signals (ESR), (2) differential pulse polarography (DPP), (3) chemiluminescence (CL), and (4) nitroblue tetrazolium reduction (NBT). All tests yield results identical with those obtained with authentic ·O-2. Extraction of ·O-2 is enhanced by tetrabutyl ammonium ion, and is maximal at 1-3 min. These results raise the possibility that substantial amounts of ·O-2 are normally sequestered in protective membranous sites in vivo.
Zusätzliches Material:
4 Ill.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1002/bio.1170060206
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