ISSN:
1573-9368
Keywords:
transgene
;
expression
;
β-lactoglobulin
;
transcription
;
translation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Many transgenes, particularly those comprising cDNA sequences fail to be expressed when they are introduced into transgenic mice. We have previously shown that this problem can be overcome in the mammary gland by co-integrating a poorly expressed cDNA transgene, comprising the sheep β- lactoglobulin promoter, with the efficiently expressed, unmodified β-lactoglobulin gene. In this report we demonstrate that the transcription of the β-lactoglobulin gene is associated with this effect because co-integration with a non-transcribed β-lactoglobulin gene fails to rescue expression. By contrast, co-integration with a translationally inactivated β-lactoglobulin transgene does rescue the expression of the second gene, but without the co-production of β-lactoglobulin protein
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1018444830518
Permalink