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  • 1
    Electronic Resource
    Electronic Resource
    Characterization of the termini of linear plasmids from Nectria haematococca and their use in construction of an autonomously replicating transformation vector (1989)
    Springer
    Current genetics 16 (1989), S. 187-194 
    Details
    ISSN: 1432-0983
    Keywords: Fusarium solani ; Mitochondrial plasmids ; Terminal inverted repeats ; Terminal proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The mitochondria of isolate FS37 from Nectria haematococca mating population I (Fusarium solani f. sp. cucurbitae) contain two linear plasmids, pFSCI and pFSC2, of 9.2 and 8.3 kbp, respectively. Evidence for a protein blocking the 5′ termini of these plasmids was obtained from exonuclease digestion experiments. A single protein band with an apparent Mr of 80 K was labeled when the DNA-protein complex of either plasmid was reacted with [125I] Bolton-Hunter reagent and then digested with DNase I. DNA sequence analysis of the termini of both plasmids revealed long inverted repeats of 1,211 by (pFSC1) and 1,027bp (pFSC2). No sequence similarity was found between the terminal inverted repeats (TIRs) of pFSC1 and pFSC2, nor was any similarity identified between the TIRs of the these plasmids and sequences of TIRs from other linear DNAs. A restriction fragment containing the TIR of pFSCI conferred autonomous replication when incorporated into an integrative transformation vector of Ustilago maydis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391476
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Characterization of siderophores from Ustilago maydis (1989)
    Springer
    Mycopathologia 108 (1989), S. 125-133 
    Details
    ISSN: 1573-0832
    Keywords: ferrichrome ; ferrichrome A ; hydroxamate ; siderophore and Ustilago maydis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Two siderophores, ferrichrome and ferrichrome A, were found in cultures of Ustilago maydis (DC) Corda. Both siderophores were found intracellularly and extracellularly. Their authenticity was confirmed by thin layer chromatography, HPLC, UV-visible spectrometry, paper electrophoresis, amino acid analysis, NMR and fast atom bombardment mass spectroscopy. Regulation of siderophore production by iron was examined. Repression of biosynthesis of extracellular siderophores occurred at 10−5 M iron. Ferrichrome was found intracellularly at all iron concentrations employed; in general, ferrichrome A was not found to be cell-associated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00436063
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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